Baran Ayşe, Keskin Cumali, Baran Mehmet Fırat, Huseynova Irada, Khalilov Rovshan, Eftekhari Aziz, Irtegun-Kandemir Sevgi, Kavak Deniz Evrim
Department of Biology, Graduate Education Institute, Mardin Artuklu University, Mardin, Turkey.
Department of Medical Services and Techniques, Vocational Higher School of Healthcare Studies, Mardin Artuklu University, Mardin, Turkey.
Bioinorg Chem Appl. 2021 Nov 30;2021:2058149. doi: 10.1155/2021/2058149. eCollection 2021.
Metallic nanoparticles are valuable materials and have a range of uses. Nanoparticles synthesized from plant wastes by environment-friendly methods have attracted the attention of researchers in recent years. Also, the advantages of biological resources and synthesis methods are attracting attention. In this study, silver nanoparticles were synthesized from fruit peels using ecofriendly method steps. The characterization of the particles obtained was determined by using a UV-visible spectrophotometer (UV-Vis.), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction diffractometer (XRD), Fourier scanning electron microscope (FESEM), and transmission electron microscopy (TEM). The nanoparticles showed maximum absorbance at 463 nm, measuring 11.61 in crystal nanosize, and presented spherical in appearance. An antimicrobial activity test was determined with the minimum inhibition concentration (MIC) method. The nanoparticles showed promising inhibitory activity on the Gram-positive and Gram-negative pathogen microorganisms ( ATCC25922, ATCC29213, ATCC11774, ATCC27833 bacteria, and yeast) at low concentrations. The cytotoxic and growth inhibitory effects of silver nanoparticles on different cancer cell lines were examined via the MTT assay.
金属纳米颗粒是有价值的材料，有一系列用途。近年来，通过环保方法从植物废料中合成的纳米颗粒引起了研究人员的关注。此外，生物资源和合成方法的优势也备受关注。在本研究中，采用环保方法步骤从果皮中合成了银纳米颗粒。使用紫外可见分光光度计（UV-Vis.）、傅里叶变换红外光谱仪（FTIR）、X射线衍射仪（XRD）、场发射扫描电子显微镜（FESEM）和透射电子显微镜（TEM）对所得颗粒进行表征。纳米颗粒在463nm处显示出最大吸光度，晶体纳米尺寸为11.61，外观呈球形。采用最低抑菌浓度（MIC）法进行抗菌活性测试。这些纳米颗粒在低浓度下对革兰氏阳性和革兰氏阴性病原微生物（ATCC25922、ATCC29213、ATCC11774、ATCC27833细菌和酵母）显示出有前景的抑制活性。通过MTT法检测了银纳米颗粒对不同癌细胞系的细胞毒性和生长抑制作用。
