猫（Felis catus）中的人畜共患病和物种及基因型调查。
Investigation of Zoonotic and Species and Genotypes in Cats (Felis catus).
机构信息
Erciyes University Faculty of Veterinary Medicine, Department of Parasitology, Kayseri, Turkey
Ondokuz Mayıs University Faculty of Veterinary Medicine, Department of Internal Medicine, Samsun, Turkey
出版信息
Turkiye Parazitol Derg. 2021 Dec 2;45(4):252-256. doi: 10.4274/tpd.galenos.2021.46320.
OBJECTIVE
and spp. are important zoonotic protozoan parasites that infect humans and various animals. We investigated the occurrence of and spp. infection in cats. To provide data on the zoonotic transmission dynamics of these parasites, genotypes of the detected isolates were investigated through DNA sequence characterization.
METHODS
A total of 100 fecal samples were collected from cats between June and October 2020 in Kayseri and Samsun provinces. Fecal samples were examined by nested polymerase chain reaction (PCR), targeting the gene of and small subunit (SSU) rRNA gene of spp. All PCR products were sequenced for genotyping.
RESULTS
Of the samples examined, Giardia intestinalis was determined in 8 samples (8.0%), whereas none of the samples were found positive for spp. Sequence analyses of the β-giardin PCR products indicated that all isolates were classed into the zoonotic assemblage B.
CONCLUSION
This study adds to the current data on the molecular epidemiology of cryptosporidiosis and giardiasis in cats. The findings also highlight the potential risk of cats for public health concerning the zoonotic transmission dynamics of .
目的
和 spp. 是重要的人畜共患的原生动物寄生虫，感染人类和各种动物。我们调查了猫感染 和 spp. 的情况。为了提供有关这些寄生虫的人畜共患病传播动力学的数据，通过 DNA 序列特征分析研究了检测到的分离株的基因型。
方法
2020 年 6 月至 10 月间，从开塞利省和萨姆松省的猫中采集了 100 份粪便样本。通过巢式聚合酶链反应 (PCR) 检测粪便样本，针对 和 spp. 的 SSU rRNA 基因的 β-微管蛋白基因。对所有 PCR 产物进行测序以进行基因分型。
结果
在检查的样本中，确定 8 份样本（8.0%）中存在肠道贾第虫，而 spp. 则均为阴性。β-微管蛋白 PCR 产物的序列分析表明，所有 分离株均归入动物群 B。
结论
本研究增加了关于猫隐孢子虫病和贾第虫病分子流行病学的现有数据。研究结果还强调了猫对公共卫生的潜在风险，涉及 的人畜共患病传播动力学。
Zotero 插件