State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, China.
Front Cell Infect Microbiol. 2022 Jul 11;12:949773. doi: 10.3389/fcimb.2022.949773. eCollection 2022.
The zoonotic protozoa parasites spp. and infect a wide range of hosts, including humans. Pigs are reservoir hosts for spp. and , which can transmit cryptosporidiosis and giardiasis to humans and other animals. The current study sought to investigate the infection rates and species/genotypes of spp. and in pigs in Hubei of China. The nested PCR and sequence analyses of the small ribosomal subunit RNA ( rRNA) gene for spp. and the β-giardin (bg) gene for was utilized to screen for the infection of those parasites in a total of 826 fresh fecal samples. Both spp. and infection rates were 0.97% (8/826). Of the genotyped positive isolates, 6/8 (75%) were and 2/8 (25%) were , while two zoonotic species assemblage E and assemblage A were also detected in 7/8 (87.5%) isolates and 1/8 (12.5%) isolates, respectively. The findings suggest that both of those parasites in pig in intensive farms of Hubei province, China, pose a potential public health risk.
人畜共患的原生动物寄生虫 spp. 和 感染范围广泛的宿主，包括人类。猪是 spp. 和 的储存宿主，它们可以将隐孢子虫病和贾第虫病传播给人类和其他动物。本研究旨在调查中国湖北省猪中 spp. 和 的感染率和种/基因型。采用巢式 PCR 和小核糖体亚单位 RNA(rRNA)基因序列分析，对 spp. 的 和 的 β-微管蛋白(bg)基因进行了筛选，共检测了 826 份新鲜粪便样本。 spp. 和 的感染率分别为 0.97%(8/826)。在基因分型阳性的分离株中，6/8(75%)为 ，2/8(25%)为 ，同时还在 7/8(87.5%)的分离株和 1/8(12.5%)的分离株中检测到两种人畜共患种 群 E 和 群 A。研究结果表明，湖北省集约化养殖场的这些寄生虫对人类健康构成潜在的公共卫生风险。
