Souna Djahida, Drissi Mourad, Almahmoud Iyad, Maurin Max
Department of Biology, Faculty of Science of Nature and Life, University of Hassiba Ben Bouali Chlef, Chlef, Algeria.
Laboratory of Molecular Biology, Genomic and Bioinformatic, Team 1: Microbial Genomics and Molecular Epidemiology of Infectious Diseases, University of Hassiba Ben Bouali Chlef, Chlef, Algeria.
Microb Drug Resist. 2022 Mar;28(3):346-354. doi: 10.1089/mdr.2020.0535. Epub 2021 Dec 10.
We evaluated the β-lactam resistance phenotypes of clinical and environmental strains of the (ECC) isolated from three Algerian hospitals. The first combination of API 20E, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and hsp60 genetic clustering methodologies were carried out for the identification of ECC strains. Our research showed that API 20E and MALDI TOF MS are satisfactory in genus identification of ECC strains, but sequence-based methods are then necessary to discriminate the species and subspecies levels. Among 36 ECC strains, 94.44% belonged to species. Twenty-five isolates clustered with the reference strain of subsp. , making it the most frequently isolated subspecies. was found only once (2.77%). All ECC isolates were phenotypically extended-spectrum β-lactamase (ESBL) producers and were resistant to ticarcillin, piperacillin, cefoxitin, cefotaxime, ceftazidime, ceftriaxone, and aztreonam, but susceptible to ertapenem and imipenem. The genetic analyses only allowed the detection of resistance genes of the group (32 strains, 88.9%), including (30 strains), (1 strain), and (1 strain). We report for the first time the detection of CTX-M-22 among ECC strains in an Algerian hospital (Tlemcen hospital). None of the isolated strains harbored , or group genes. In this review, we address recent comparison in the identification methods of multidrug-resistant in Algeria, focusing also on the CTX-M ESBLs. This represents a serious public health challenge, which requires the clarification of the current situation and warrants the reinforcement of hygiene measures in the Algerian hospitals.
我们评估了从阿尔及利亚三家医院分离出的肠杆菌科临床菌株和环境菌株的β-内酰胺耐药表型。首先采用API 20E、基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）和hsp60基因聚类方法对肠杆菌科菌株进行鉴定。我们的研究表明，API 20E和MALDI TOF MS在肠杆菌科菌株的属鉴定中令人满意，但需要基于序列的方法来区分种和亚种水平。在36株肠杆菌科菌株中，94.44%属于某一物种。25株分离株与某亚种的参考菌株聚类，使其成为最常分离的亚种。某菌株仅被发现一次（2.77%）。所有肠杆菌科分离株在表型上均为超广谱β-内酰胺酶（ESBL）产生菌，对替卡西林、哌拉西林、头孢西丁、头孢噻肟、头孢他啶、头孢曲松和氨曲南耐药，但对厄他培南和亚胺培南敏感。基因分析仅检测到某组的耐药基因（32株，88.9%），包括某基因（30株）、某基因（1株）和某基因（1株）。我们首次在阿尔及利亚一家医院（特莱姆森医院）的肠杆菌科菌株中检测到CTX-M-22。分离出的菌株均未携带某、某或某组基因。在本综述中，我们讨论了阿尔及利亚多重耐药肠杆菌科鉴定方法的最新比较，同时也关注CTX-M型ESBLs。这是一个严峻的公共卫生挑战，需要厘清当前状况，并加强阿尔及利亚医院的卫生措施。
