Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), University Podiatric Clinic, Complutense University of Madrid, Madrid, Spain.
Microbiology Section, Medical Faculty of the Complutense University of Madrid, Madrid, Spain.
J Med Virol. 2022 Jun;94(6):2897-2901. doi: 10.1002/jmv.27514. Epub 2021 Dec 15.
The methods that are used for the diagnostic confirmation of human papillomavirus (HPV) include excisional biopsy and histopathological studies or polymerase chain reaction (PCR). They are invasive, laborious, and subject to ethical restrictions due to the benign nature of these warts. This study aims to analyse the accuracy of noninvasive swab samples to diagnose plantar warts. Fifty plantar warts were included in the study. Skin swabs and hyperkeratosis skin scales were collected from each wart. Multiplex PCR was performed to detect and type the HPVs. The prevalence of HPV in this study was 90% when the sample was obtained using the wart scraping method and 94% when it was obtained using swabs and the new method. In 45 of the 45 positive samples (sensitivity: 100%), the result between the wart scab and wart swab were almost identical. The genotyping result was identical in all 46 patients who had a positive result using both methods. The swab method appears to be a simple and accurate technique to diagnose plantar warts due to HPV. It is a noninvasive technique that could be performed even by inexperienced professionals and in patients with pain or a fear of needles.
用于诊断人乳头瘤病毒（HPV）的方法包括切除活检和组织病理学研究或聚合酶链反应（PCR）。这些方法具有侵袭性、繁琐且受到伦理限制，因为这些疣通常是良性的。本研究旨在分析非侵入性拭子样本诊断足底疣的准确性。本研究纳入了 50 例足底疣。从每个疣中采集皮肤拭子和过度角化的皮肤鳞屑。采用多重 PCR 检测和分型 HPV。当使用疣刮取法获得样本时，本研究中 HPV 的患病率为 90%，当使用拭子和新方法获得样本时，HPV 的患病率为 94%。在 45 例阳性样本中（敏感性：100%），疣刮屑和疣拭子之间的结果几乎完全相同。两种方法均呈阳性的 46 例患者的基因分型结果完全一致。由于 HPV 引起的足底疣，拭子法似乎是一种简单而准确的诊断方法。它是一种非侵入性技术，即使是没有经验的专业人员也可以进行，并且可以在有疼痛或害怕针的患者中进行。
