Kradin R L, McCarthy K M, Preffer F I, Schneeberger E E
J Leukoc Biol. 1986 Oct;40(4):407-17. doi: 10.1002/jlb.40.4.407.
Alveolar macrophages (AM) from adult and newborn rats were studied by flow cytometry and ultrastructural morphometry. We observed that the laser scatter and autofluorescent properties of newborn macrophages were different from those of adult cells. Relative to the adult AM, the forward-angle laser scatter obtained with the newborn AM was reduced; this optical measurement appeared to correlate with the smaller mean size, as determined by ultrastructural and electronic volume measurements. The diminished right-angle laser scatter (90 degrees angle) correlated with the presence of fewer small, irregularly shaped lysosomal structures in the newborn AM, compared with AM from adult animals. AM from 1-2-day-old rats displayed large vacuoles containing multilamellar structures, which proved to be less effective at scattering light. Cells from newborn rats were less autofluorescent, a finding that appeared to correlate best with the numbers of secondary lysosomes. Flow cytometry may be used to discern structural alterations that occur during the maturation of AM. These changes correlate well with quantitative ultrastructural analyses of these cells.
通过流式细胞术和超微结构形态计量学研究了成年大鼠和新生大鼠的肺泡巨噬细胞（AM）。我们观察到，新生巨噬细胞的激光散射和自发荧光特性与成年细胞不同。相对于成年AM，新生AM获得的前向角激光散射减少；这种光学测量似乎与超微结构和电子体积测量确定的较小平均尺寸相关。与成年动物的AM相比，新生AM中直角激光散射（90度角）的减少与较小的、形状不规则的溶酶体结构数量较少有关。1-2日龄大鼠的AM显示出含有多层结构的大液泡，这些液泡在散射光方面效果较差。新生大鼠的细胞自发荧光较弱，这一发现似乎与次级溶酶体的数量最相关。流式细胞术可用于识别AM成熟过程中发生的结构改变。这些变化与这些细胞的定量超微结构分析密切相关。
