利用骨髓来源的巨噬细胞研究巨噬细胞极化。
Investigation of macrophage polarization using bone marrow derived macrophages.
作者信息
Ying Wei, Cheruku Patali S, Bazer Fuller W, Safe Stephen H, Zhou Beiyan
机构信息
Department of Animal Science, Texas A&M University, TX, USA.
出版信息
J Vis Exp. 2013 Jun 23(76):50323. doi: 10.3791/50323.
Abstract
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
摘要
这篇文章描述了一种易于构建的适应性体外模型，用于研究巨噬细胞极化。在GM-CSF/M-CSF存在的情况下，骨髓中的造血干细胞/祖细胞被诱导进行单核细胞分化，随后进行M1或M2刺激。激活状态可以通过细胞表面抗原、基因表达和细胞信号通路的变化来追踪。
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