Klingemann H G, Ebert J, Deeg H J
J Leukoc Biol. 1986 Oct;40(4):491-5. doi: 10.1002/jlb.40.4.491.
We examined T-cells, B-cells, and natural killer cells of four normal individuals for surface-bound fibronectin (FN) using fluorescence-activated cell sorter analysis and double fluorescence labeling with monoclonal antibodies. While Leu 12 (CD 19)-positive B-cells stained also uniformly with anti-FN antibody, neither OKT 3 (CD 3)-positive T-cells nor Leu 11 (CD 16)-positive natural killer cells could be labeled with anti-FN. Although an anti-FN receptor antibody was not available, these data strongly suggest a distinct pattern of FN-binding by human lymphocytes.
我们使用荧光激活细胞分选分析和单克隆抗体双荧光标记，检测了四名正常个体的T细胞、B细胞和自然杀伤细胞表面结合的纤连蛋白（FN）。虽然Leu 12（CD 19）阳性B细胞也能被抗FN抗体均匀染色，但OKT 3（CD 3）阳性T细胞和Leu 11（CD 16）阳性自然杀伤细胞均不能被抗FN标记。尽管没有抗FN受体抗体，但这些数据强烈提示人类淋巴细胞结合FN的模式不同。
