State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China; Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control, Ningbo Urban Environment Observation and Research Station-NUEORS, Institute of Urban Environment, Chinese Academy of Sciences, Ningbo 315800, China.
Sci Total Environ. 2022 Feb 25;809:152184. doi: 10.1016/j.scitotenv.2021.152184. Epub 2021 Dec 7.
Plastic mulching is a common practice in agricultural systems and is often combined with fertilization. Biodegradable plastics (BPs) are becoming an alternative to non-biodegradable plastics (non-BPs) for soil mulching. However, the effects of fertilization on the microbial communities on BPs remain unclear. Here, we explored the responses of the plastisphere to different fertilization practices in soil-based microcosms containing three BPs: polylactic acid (PLA), poly (butylene succinate) (PBS), and poly (butylene-adipate-co-terephthalate) (PBAT), and one non-BP (low-density polyethylene, LDPE). The 16S and ITS rRNA gene-based Illumina sequencing method were used to identify the bacterial and fungal communities on the plastics and in the soils. Microbial community structure on BPs was significantly different from that in soils and on LDPE. The predicted functional profiles of bacteria on BPs, especially PBAT, were distinct from those in soils. The plastisphere communities on BPs were dominated by microbes adapted to access and utilize carbon sources compared with of the communities on LDPE. Application of manure increased the alpha diversity of bacterial communities on BPs but decreased it on LDPE. The structure of bacterial communities on BPs changed with the application of manure. Our research establishes the baseline dynamics of plastisphere communities on BPs in soils.
塑料地膜覆盖是农业系统中的一种常见做法，通常与施肥相结合。可生物降解塑料（BPs）正逐渐成为土壤覆盖用不可生物降解塑料（non-BPs）的替代品。然而，施肥对 BP 上微生物群落的影响尚不清楚。在这里，我们在含有三种 BP（聚乳酸（PLA）、聚丁二酸丁二醇酯（PBS）和聚丁二酸丁二醇酯-己二酸酯-对苯二甲酸酯（PBAT）和一种 non-BP（低密度聚乙烯，LDPE）的土壤微宇宙中，探索了不同施肥措施对塑料地膜覆盖物上的原质体的影响。使用基于 16S 和 ITS rRNA 基因的 Illumina 测序方法来鉴定塑料上和土壤中的细菌和真菌群落。BP 上的微生物群落结构与土壤和 LDPE 上的微生物群落结构有显著差异。BP 上细菌的预测功能图谱，尤其是 PBAT，与土壤中的图谱明显不同。与 LDPE 相比，BP 上的原质体群落由适应获取和利用碳源的微生物主导。施用有机肥增加了 BP 上细菌群落的 alpha 多样性，但降低了 LDPE 上的 alpha 多样性。BP 上细菌群落的结构随有机肥的施用而发生变化。我们的研究确立了土壤中 BP 上原质体群落的基线动态。
