School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
Sustainable Development Study Centre (SDSC), Government College University, Lahore, Pakistan.
Bioresour Technol. 2022 Feb;346:126513. doi: 10.1016/j.biortech.2021.126513. Epub 2021 Dec 7.
Advances in microbial enzyme technology offer a significant opportunity for developing low-energy bioconversion solutions for industrial wastes as inexpensive feedstocks for useful products. In this short communication, two agro-food industrial wastes, chicken feather powder (CFP) and okara, were converted into peptides by a Bacillus licheniformis mutant using solid-state fermentation (SSF). The optimum SSF conditions for okara to CFP ratio, inoculum size, and time were 0.7 (7:10), 15%, and 90 h, respectively, which produced 185.99 mg/g peptides, with 910.12 U/g keratinase activity and 85.03% antioxidant scavenging activity. Compared to okara, CFP with mutant strain showed 11.28% higher keratinase activity and produced higher amounts of peptides (5.51%).
微生物酶技术的进步为开发低能耗的生物转化解决方案提供了重要机会，可将工业废物转化为廉价原料，用于生产有用产品。在本简讯中，利用固态发酵（SSF），枯草芽孢杆菌突变株将两种农业食品工业废物——鸡毛粉（CFP）和豆渣——转化为肽。豆渣与 CFP 的最佳 SSF 条件比、接种量和时间分别为 0.7（7:10）、15%和 90 h，分别产生 185.99 mg/g 肽，角蛋白酶活性为 910.12 U/g，抗氧化清除活性为 85.03%。与豆渣相比，突变株处理的 CFP 表现出 11.28%更高的角蛋白酶活性，产生更多的肽（5.51%）。
