Key Laboratory of Photoelectric Materials and Devices of Zhejiang Province, Ningbo University, Ningbo, 315211, China; Engineering Research Center for Advanced Infrared Photoelectric Materials and Devices of Zhejiang Province, Ningbo University, Ningbo, 315211, China.
School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China.
Biosens Bioelectron. 2022 Mar 1;199:113860. doi: 10.1016/j.bios.2021.113860. Epub 2021 Dec 2.
The rapid and reliable detection of bacteria plays an important role in clinical and veterinary practice. A stable, label free, compact, and sensitive long-period fiber grating (LPFG) sensor based on egg yolk antibody (IgY) was proposed for the detection of Staphylococcus aureus (S. aureus). LPFG was fabricated with laser writing technology, and specific IgY was further immobilized on the grating region and then combined with the corresponding bacteria. S. aureus was detected by tracking the change of resonance wavelength in the LPFG transmission spectrum caused by bacteria-antibody interaction induced by the increase of biological cover thickness and density after the immune reaction. The testing results showed that the proposed sensor was selective and sensitive to S. aureus measurement, and the detection limit is approximately 33 CFU/ml. The proposed antibody immobilization method is very simple, and the optical fiber can be manufactured in batch to reduce the cost. The detection time of the sensor is around 20 min, which is fast and suitable for detection. The assay was successfully applied for the quantitative analysis of S. aureus in natural waters and met the needs of on-site screening trace pathogenic bacteria in food safety control.
细菌的快速可靠检测在临床和兽医实践中起着重要作用。本研究提出了一种基于卵黄抗体（IgY）的稳定、无标记、紧凑和灵敏的长周期光纤光栅（LPFG）传感器，用于检测金黄色葡萄球菌（S. aureus）。采用激光写入技术制作 LPFG，并进一步将特异性 IgY 固定在光栅区域，然后与相应的细菌结合。通过跟踪免疫反应后生物覆盖厚度和密度增加引起的细菌-抗体相互作用导致 LPFG 传输光谱中共振波长的变化来检测 S. aureus。测试结果表明，该传感器对 S. aureus 的测量具有选择性和敏感性，检测限约为 33 CFU/ml。所提出的抗体固定方法非常简单，并且可以批量制造光纤以降低成本。传感器的检测时间约为 20 分钟，速度快，适合检测。该测定法成功地应用于天然水中金黄色葡萄球菌的定量分析，满足了食品安全控制中现场筛选痕量致病菌的需要。
