Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang province, 315211, China.
College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
Analyst. 2024 Jan 29;149(3):876-884. doi: 10.1039/d3an01564e.
Specific and rapid detection of live () in environmental and food samples is critically important for protecting human health. In order to fulfill this purpose, two kinds of novel egg yolk antibody (IgY) immobilized immunomagnetic beads (IMBs; mSiO-IgY and mMOF-IgY), with core-shell mSiO and mMOF as substrate, were prepared for selectively enriching from samples. Furthermore, the IMBs with captured were collected and re-dissolved in 0.5 mL PBS. After that, a cotton swab coated with sodium dodecylsulfate (SDS) was put in the solution to lyse cells and emit ATP bioluminescence of the luciferin/luciferase system. Finally, a portable bioluminescence detector was used for quantification of ATP corresponding to concentration. The results demonstrated that mMOF-IgY can enrich more than mSiO-IgY and emit a stronger signal. The reasons may be due to the higher immobilization amount of IgY on the IMBs. Under optimal conditions, the calibration line of concentration was 10-10 CFU mL by mMOF-IgY within 30 min. The low detection limit of was 3 CFU mL. The results demonstrated that the assay takes much shorter time than plate counting. Its portability and excellent detection capability are suitable for rapid monitoring of specific pathogens in foods.
特异性和快速检测环境和食品样本中的（）对于保护人类健康至关重要。为此，我们制备了两种新型的卵黄抗体（IgY）固定化免疫磁珠（IMB；mSiO-IgY 和 mMOF-IgY），以具有核壳结构的 mSiO 和 mMOF 作为基质，用于从样品中选择性富集（）。此外，用捕获（）的 IMB 收集并重新溶解在 0.5 mL PBS 中。然后，将涂有十二烷基硫酸钠（SDS）的棉签放入溶液中裂解（）细胞并发出荧光素/荧光素酶系统的 ATP 生物发光。最后，使用便携式生物发光检测仪定量与（）浓度相对应的 ATP。结果表明，mMOF-IgY 可以比 mSiO-IgY 富集更多的（）并发出更强的信号。原因可能是因为 IMB 上的 IgY 固定量更高。在最佳条件下，mMOF-IgY 在 30 分钟内可将（）浓度的校准线范围设定为 10-10 CFU mL。（）的最低检测限为 3 CFU mL。结果表明，该检测方法比平板计数法耗时更短。其便携性和出色的检测能力非常适合快速监测食品中的特定病原体。
