School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai, 264005, PR China.
School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai, 264005, PR China; Yantai Center for Food and Drug Control, Yantai, 264000, PR China.
Talanta. 2022 Mar 1;239:123115. doi: 10.1016/j.talanta.2021.123115. Epub 2021 Nov 30.
Nowadays, molecularly imprinted polymers (MIPs) coated silica stationary phases (SPs) have aroused great attention, owing to their good properties of high selectivity, good stability, facile synthesis procedure and low cost. In this study, zidovudine imprinted polymers coated silica stationary phases (MIPs/SiO SPs) were synthesized by surface imprinting technique using zidovudine as the template molecule, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linking agent, azobisisobutyronitrile as the initiator, and bare silica spheres (particle size, 5 μm; pore size, 20 nm) as substrates. In the process, reagents with low concentration were used to prepare thin layer of MIPs coating on the surface of silica microbeads. The properties of the materials were characterized by scanning electron microscope (SEM), fourier transform infrared spectrometer (FT-IR), carbon elemental analysis and N adsorption-desorption experiment. The obtained SPs were packed into stainless steel columns (2.1 mm × 150 mm) via a slurry method. The prepared columns were applied for separation of nucleoside analogues with similar chemical structures and strong polarity. The retention mechanism of MIPs/SiO SPs for nucleoside analogues was investigated carefully. And the chromatographic performances of the resulting MIPs based SPs were superior to those of the commercial SPs. Furthermore, the synthesized MIPs/SiO SPs possessed great potentials in separation of ginsenosides. This investigation demonstrated that MIPs based SPs were successfully synthesized and provided a new approach to polar compounds separation and analysis.
如今，基于印迹聚合物（MIPs）的二氧化硅固定相（SPs）因其高选择性、良好的稳定性、简单的合成程序和低成本等优点而受到广泛关注。在这项研究中，采用表面印迹技术，以齐多夫定为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂、偶氮二异丁腈为引发剂，以粒径为 5μm、孔径为 20nm 的裸二氧化硅球为基底，合成了齐多夫定印迹聚合物二氧化硅固定相（MIPs/SiO SPs）。在这个过程中，使用低浓度的试剂在二氧化硅微球表面制备了一层很薄的 MIPs 涂层。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、元素碳分析和 N 吸附-脱附实验对材料的性能进行了表征。通过浆料法将得到的 SPs 填充到不锈钢柱（2.1mm×150mm）中。制备的柱子被用于分离具有相似化学结构和强极性的核苷类似物。仔细研究了 MIPs/SiO SPs 对核苷类似物的保留机制。与商业 SPs 相比，所得 MIPs 基 SPs 的色谱性能更优。此外，合成的 MIPs/SiO SPs 在分离人参皂苷方面具有很大的潜力。这项研究表明，成功合成了基于印迹聚合物的 SPs，并为极性化合物的分离和分析提供了一种新方法。
