Chai Peijun, Geng Xuhui, Zhu Ruirui, Wu Wenpu, Wang Xuesong, Li Jinhua, Fu Longwen, Wang Hongdan, Liu Wanhui, Chen Lingxin, Song Zhihua
School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai, 264005, PR China.
Department of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Key Laboratory of Deep-sea Composition Detection Technology of Liaoning Province, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China.
Anal Chim Acta. 2023 Sep 22;1275:341611. doi: 10.1016/j.aca.2023.341611. Epub 2023 Jul 11.
Facing the difficulties in chromatographic separation of polar compounds, this investigation devotes to developing novel stationary phase. Molecularly imprinted polymers (MIPs) have aroused wide attention, owing to their outstanding selectivity, high stability, and low cost. In this work, a novel stationary phase based on carbon dots (CDs), MIP layer, and silica beads was synthesized to exploit high selectivity of MIPs, excellent physicochemical property of CDs, and outstanding chromatographic performances of silica microspheres simultaneously. The MIP doped CDs coated silica (MIP-CDs/SiO) stationary phase was systematically characterized by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) surface area measurement, and carbon elemental analysis. Furthermore, the chromatographic performance of the MIP-CDs/SiO column was thoroughly assessed by using a wide variety of compounds (including nucleosides, sulfonamides, benzoic acids, and some other antibiotics). Meanwhile, the separation efficiency of the MIP-CDs/SiO stationary phase was superior to other kinds of stationary phases (e.g. nonimprinted NIP-CDs/SiO, MIP/SiO, and C18-SiO). The results demonstrated that MIP-CDs/SiO column exhibited best performance in terms of chromatographic separation. For all tested compounds, the resolution value was not less than 1.60, and the column efficiency of MIP-CDs/SiO for thymidine was 22,740 plates/m. The results further indicate that the MIP-CDs/SiO column can combine the good properties of MIP, CDs, with those of silica microbeads. Therefore, the developed MIP-CDs/SiO stationary phase can be applied in the separation science and chromatography-based techniques.
面对极性化合物色谱分离中的困难，本研究致力于开发新型固定相。分子印迹聚合物（MIPs）因其出色的选择性、高稳定性和低成本而备受关注。在这项工作中，合成了一种基于碳点（CDs）、MIP层和硅胶珠的新型固定相，以同时利用MIPs的高选择性、CDs优异的物理化学性质和硅胶微球出色的色谱性能。通过扫描电子显微镜（SEM）、布鲁诺尔-埃米特-泰勒（BET）表面积测量和碳元素分析对MIP掺杂CDs包覆硅胶（MIP-CDs/SiO）固定相进行了系统表征。此外，使用多种化合物（包括核苷、磺胺类、苯甲酸和其他一些抗生素）全面评估了MIP-CDs/SiO柱的色谱性能。同时，MIP-CDs/SiO固定相的分离效率优于其他类型的固定相（如非印迹NIP-CDs/SiO、MIP/SiO和C18-SiO）。结果表明，MIP-CDs/SiO柱在色谱分离方面表现出最佳性能。对于所有测试化合物，分离度值不小于1.60，MIP-CDs/SiO对胸苷的柱效为22740理论塔板数/米。结果进一步表明，MIP-CDs/SiO柱可以将MIP、CDs的良好性能与硅胶微球的性能结合起来。因此，所开发的MIP-CDs/SiO固定相可应用于分离科学和基于色谱的技术中。
