Department of Biology, Qom Branch, Islamic Azad University, Iran.
Department of Microbiology, Damghan Branch, Islamic Azad University, Iran.
J Burn Care Res. 2022 Jul 1;43(4):951-956. doi: 10.1093/jbcr/irab229.
Burns are the most prevalent type of trauma in the world, and they have a high fatality rate. For cutaneous wound healing, modern and natural therapies, particularly probiotic supplements, have lately been considered. The goal of this study was to see how Lactiplantibacillus plantarum affected wound healing as well as the antibacterial activity of probiotic lactobacilli against Pseudomonas aeruginosa. The glass slide method was used to assess anti-adhesion activity, and the high-performance liquid chromatography method was used to quantify anti-adhesion chemicals in cell-free supernatant. Multidrug-resistant (MDR) P. aeruginosa was administered subcutaneously directly on the burn after induction of second-degree wounds. Three groups of animals were created. Every day, the supernatants were sprayed for therapy, and the wound healing was monitored. Lactobacilli bacteria had good anti-adhesion effects on P. aeruginosa, according to our findings, and high-performance liquid chromatography research revealed that their inhibitory effect could be attributable to four main organic acids: lactic acid, acetic acid, citric acid, and succinic acid. When the effect of treatments on fibroblastic cells was examined, it was discovered that the group treated with L. plantarum supernatants had the most fibroblastic cells when compared to the nontreated group. Furthermore, the bacteria increased the number of fibroblastic cells, re-epithelialization in the wound area, and the thickness of the epidermis and dermis layers. Lactobacilli bacteria's antimicrobial activity against MDR P. aeruginosa was determined by prevents infection. These findings revealed that L. plantarum can treat a P. aeruginosa infection in a second-degree burn and can significantly reduce inflammation.
烧伤是世界上最常见的创伤类型，其死亡率很高。对于皮肤伤口愈合，现代和自然疗法，尤其是益生菌补充剂，最近被认为是有效的方法。本研究的目的是观察植物乳杆菌对伤口愈合的影响以及益生菌乳杆菌对铜绿假单胞菌的抗菌活性。采用玻璃载玻片法评估抗黏附活性，采用高效液相色谱法定量细胞上清液中的抗黏附化学物质。将多药耐药（MDR）铜绿假单胞菌直接皮下注射到诱导的二度烧伤创面后。创建了三组动物。每天喷洒上清液进行治疗，并监测伤口愈合情况。根据我们的研究结果，发现乳酸菌对铜绿假单胞菌具有良好的抗黏附作用，高效液相色谱研究表明其抑制作用可能归因于四种主要有机酸：乳酸、乙酸、柠檬酸和琥珀酸。当研究治疗对成纤维细胞的影响时，发现与未处理组相比，用 L. plantarum 上清液处理的组中成纤维细胞数量最多。此外，细菌增加了成纤维细胞的数量，促进了伤口区域的再上皮化以及表皮和真皮层的厚度。通过预防感染，乳酸菌对 MDR 铜绿假单胞菌的抗菌活性。这些发现表明，L. plantarum 可以治疗二度烧伤中的铜绿假单胞菌感染，并能显著减轻炎症。
