Abootaleb Mohammad, Mohammadi Bandari Narjes, Arbab Soleimani Nazila
Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran.
Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Iran J Basic Med Sci. 2021 Feb;24(2):143-149. doi: 10.22038/IJBMS.2020.47447.10920.
Burns are the most common type of trauma with a high mortality rate worldwide. The use of modern and natural medicines, especially probiotic products, has been recently considered for cutaneous wound healing. The present study was designed to investigate the effect of on wound healing caused by .
In this study, the anti-adhesion activity of was examined by the glass slide method, and inhibitory substances in the cell-free supernatant (CFS) were quantified by high-performance liquid chromatography (HPLC). Following the induction of second-degree wounds, multidrug-resistant (MDR) was injected subcutaneously and directly on the burn. The animals were divided into four groups. The supernatant of was sprayed for treatment every day and wound healing was examined.
Based on our findings, the supernatant of showed considerable anti-adhesion effects on . HPLC analysis indicated that the inhibitory effect of this supernatant can be due to four main organic acids including lactic acid, acetic acid, citric acid, and succinic acid. The effect of treatment on fibroblastic cells showed that the treated group by supernatant of had more fibroblastic cells compared with the non-treated group. Moreover, this supernatant increased the rate of fibroblastic cells, re-epithelialization in the wound area, and the largest thickness of the epidermis and dermis layers.
The present findings showed that supernatant significantly reduced inflammation and could be used to treat infection in second-degree burns.
烧伤是全球最常见的创伤类型，死亡率很高。最近，人们开始考虑使用现代药物和天然药物，尤其是益生菌产品来促进皮肤伤口愈合。本研究旨在调查[具体物质]对[具体原因]所致伤口愈合的影响。
在本研究中，采用载玻片法检测[具体物质]的抗黏附活性，并用高效液相色谱法（HPLC）对无细胞上清液（CFS）中的抑制物质进行定量分析。诱导二度伤口形成后，将多重耐药（MDR）[具体物质]皮下注射并直接注射到烧伤部位。将动物分为四组。每天喷洒[具体物质]的上清液进行治疗，并检查伤口愈合情况。
根据我们的研究结果，[具体物质]的上清液对[具体物质]显示出相当大的抗黏附作用。HPLC分析表明，该上清液的抑制作用可能归因于四种主要有机酸，包括乳酸、乙酸、柠檬酸和琥珀酸。对成纤维细胞的治疗效果表明，与未治疗组相比，用[具体物质]上清液治疗的组有成纤维细胞更多。此外，该上清液提高了成纤维细胞的比例、伤口区域的再上皮化程度以及表皮和真皮层的最大厚度。
目前的研究结果表明，[具体物质]上清液可显著减轻炎症，可用于治疗二度烧伤中的[具体物质]感染。
