Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Department of Research and Development, Arka Industrial Cluster, Mashhad, Iran.
Biotechnol Appl Biochem. 2022 Dec;69(6):2540-2549. doi: 10.1002/bab.2303. Epub 2022 Jan 8.
This study was performed to compare the noncapsulated with nanoliposome-encapsulated phenolic-rich fraction (PRF) obtained from Rheum ribes as a dietary additive and to assess their health-promoting potentials in the mice infected by enteropathogenic Escherichia coli (O157:H7). Upon fractionation, the ethyl acetate fraction with 46.9 ± 2.17 mg GAE/g DW was found as a highest phenolic content. The PRF successfully loaded into nanoliposome structure with a nanometer in size (193.2 nm) and spherical shape and homogeneous dispersion. The gallic acid, salicylic acid, caffeic acid, cinnamic acid, catechin, ellagic acid, and ferulic acid are bioactive phenolics present in the nanoliposome-loaded PRF; however, the main bioactive compounds are cinnamic acid (911 μg/g DW) and ellagic acid (826 μg/g DW). The infection caused by E. coil impaired the weight gain and food intake, liver function, morpho structural characteristics of jejunum, upregulated the expression of inflammatory genes (Cox2, iNOS), downregulation of antioxidant-related genes (SOD, GPX), and increased the ileal population of E. coil. The addition of nonencapsulated PRF and nanoliposome-encapsulated PRF at the concentration of 10 mg TPC/kg BW/day improved these parameters although the nanoliposome-encapsulated PRF revealed more potential as compared with the nonencapsulated PRF in improving the health parameters in mice. The higher health-promoting activity of nanoliposome-encapsulated PRF could be associated with its enhanced intestinal absorption, bioavailability, bioaccessibility, and bioactivity. Consequently, the nanoliposome-encapsulated PRF could be considered as a promising phytobiotic against E. coil infection in mice.
本研究旨在比较大黄（Rheum ribes）中非包裹与纳米脂质体包裹的富含酚类的提取物（PRF）作为膳食添加剂，并评估其对感染肠致病性大肠杆菌（O157：H7）的小鼠的健康促进潜力。经过分离，发现乙酸乙酯部分的酚含量最高，为 46.9±2.17 mg GAE/g DW。PRF 成功负载到纳米脂质体结构中，纳米脂质体的大小为 193.2nm，呈球形且均匀分散。纳米脂质体负载的 PRF 中存在没食子酸、水杨酸、咖啡酸、肉桂酸、儿茶素、鞣花酸和阿魏酸等生物活性酚类，但主要的生物活性化合物是肉桂酸（911μg/g DW）和鞣花酸（826μg/g DW）。大肠杆菌感染会损害体重增加和食物摄入、肝功能、空肠形态结构特征，上调炎症基因（Cox2、iNOS）的表达，下调抗氧化相关基因（SOD、GPX），增加回肠大肠杆菌的数量。添加非包裹 PRF 和纳米脂质体包裹 PRF 的浓度为 10mg TPC/kg BW/天可以改善这些参数，尽管与非包裹 PRF 相比，纳米脂质体包裹 PRF 对改善小鼠健康参数更有潜力。纳米脂质体包裹 PRF 具有更高的健康促进活性，可能与其增强的肠道吸收、生物利用度、生物可及性和生物活性有关。因此，纳米脂质体包裹 PRF 可被视为一种有前途的植物生物制剂，可用于预防小鼠大肠杆菌感染。
