Department of Urology, Xiang'an Hospital of Xiamen University, Xiamen, China.
Department of Gastroenterology, Zhongshan Hospital Affiliated to Xiamen University, Fujian 361000, China.
Ecotoxicol Environ Saf. 2022 Jan 1;229:113059. doi: 10.1016/j.ecoenv.2021.113059. Epub 2021 Dec 8.
Perfluorinated compounds are emerging organic pollutants widely used in building materials, textiles, and electric equipment. Herein, silico analysis was conducted using bioinformatics approach to assess the potential relationship between bladder cancer and perfluorinated compounds. Transcriptome profiles and data of perfluorinated compounds were obtained from The Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression and Comparative Toxicogenomics databases. Gene Ontology (GO9 and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that interactive genes were mainly enriched in bladder cancer (BC). Transcriptome profiles were used to verify the expression of m6A-related genes at the mRNA and protein levels. Most m6A-related genes predicted BC prognosis. Survival analysis and ROC curves demonstrated that the expression levels of m6A-related genes were associated with BC prognosis. Perfluorooctanoic acid (PFOA) significantly increased the cell proliferation ability and promoted cell invasion capacity. In addition, PFOA significantly increased the cell viability and cell invasion capacity of T24 and BIU-87 cell lines compared with the control group. Taken together, these results show that perfluorinated compounds could promote BC progression. DATA AVAILABILITY: Data and materials are available within the manuscript.
全氟化合物是一种新兴的有机污染物，广泛应用于建筑材料、纺织品和电器设备中。本研究采用生物信息学方法进行硅分析，评估膀胱癌与全氟化合物之间的潜在关系。从癌症基因组图谱（TCGA）和基因型-组织表达和比较毒理学基因组数据库中获得全氟化合物的转录组谱和数据。基因本体论（GO9）和京都基因与基因组百科全书（KEGG）分析表明，相互作用的基因主要富集在膀胱癌（BC）中。转录组谱用于验证 m6A 相关基因在 mRNA 和蛋白质水平上的表达。大多数 m6A 相关基因预测 BC 预后。生存分析和 ROC 曲线表明，m6A 相关基因的表达水平与 BC 的预后相关。全氟辛酸（PFOA）显著增加了细胞的增殖能力，并促进了细胞的侵袭能力。此外，与对照组相比，PFOA 显著增加了 T24 和 BIU-87 细胞系的细胞活力和细胞侵袭能力。综上所述，这些结果表明全氟化合物可能促进膀胱癌的进展。数据可用性：数据和材料在本文中可用。
