Segabinazzi Lorenzo G, Friso Aime M, Correal Sebastian B, Crespilho André M, Dell'Aqua José Antonio, Miró Jordi, Papa Frederico O, Alvarenga Marco Antonio
Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - UNESP, Botucatu, Brazil.
Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Spain.
Theriogenology. 2017 Dec;104:120-126. doi: 10.1016/j.theriogenology.2017.08.007. Epub 2017 Aug 12.
Persistent mating-induced endometritis (PMIE) results in decreased fertility in horses, thereby causing a significant impact in the horse market. Platelet-rich plasma (PRP), a modulator of the inflammatory response, has been largely used in veterinary medicine. Here, we investigated the effects of PRP on uterine inflammation, conception rate, endometrial polymorphonuclear neutrophil (PMN) migration, and COX-2 protein levels in the endometrial tissue. Thirteen PMIE-susceptible mares were used for artificial insemination (AI). The mares were inseminated with fresh semen in three consecutive cycles in a cross-over study design. The following cycle classifications were used: control cycle, no pharmacological interference; pre-AI, 20 mL of PRP was infused 24 h before AI; and post-AI, 20 mL of PRP was infused four h after AI. Follicular dynamics were monitored daily by transrectal ultrasound. When a follicle larger than 35 mm was detected, ovulation was induced with deslorelin acetate (1 mg, im). AI was performed 24 h after ovulation induction. Intrauterine fluid (FLU) was evaluated by ultrasonography before and 24 h after AI. PMNs in uterine cytology (CYT) and biopsy (HIS) were also observed before and 24 h after AI. Pregnancy was determined within 14 days after ovulation. Number of COX-2 positive cells was evaluated by immunohistochemistry. Both PRP treatments resulted in a decrease of PMNs in the CYT after breeding when compared to controls. FLU did not differ between cycles; however, the conception rates were significantly higher in the PRP mares. Mares positive for endometritis decreased in both treatment groups, and a more intense positive COX-2 labeling was observed in the control group when compared to the two treatment groups. In conclusion, PRP beneficially reduces inflammatory response in PMIE mares independent of when treatments were administered, thus increasing chances of successful pregnancy.
持续性交配诱导性子宫内膜炎（PMIE）会导致母马生育力下降，从而对马匹市场产生重大影响。富含血小板血浆（PRP）作为一种炎症反应调节剂，已在兽医学中广泛应用。在此，我们研究了PRP对子宫炎症、受孕率、子宫内膜多形核中性粒细胞（PMN）迁移以及子宫内膜组织中COX - 2蛋白水平的影响。选用13匹易患PMIE的母马进行人工授精（AI）。在交叉研究设计中，母马在三个连续周期内用新鲜精液进行授精。采用以下周期分类：对照周期，无药物干预；AI前，在AI前24小时注入20 mL PRP；AI后，在AI后4小时注入20 mL PRP。每天通过经直肠超声监测卵泡动态。当检测到卵泡大于35 mm时，用醋酸地索高诺酮（1 mg，肌肉注射）诱导排卵。在排卵诱导后24小时进行AI。在AI前和AI后24小时通过超声检查评估子宫内液体（FLU）。在AI前和AI后24小时还观察子宫细胞学（CYT）和活检（HIS）中的PMN。在排卵后14天内确定是否怀孕。通过免疫组织化学评估COX - 2阳性细胞数量。与对照组相比，两种PRP处理均导致配种后CYT中的PMN减少。各周期之间FLU无差异；然而，PRP处理的母马受孕率显著更高。两个治疗组中患子宫内膜炎的母马数量均减少，与两个治疗组相比，对照组中观察到更强烈的COX - 2阳性标记。总之，PRP可有效降低PMIE母马的炎症反应，且与给药时间无关，从而增加成功受孕的机会。
