Laboratory of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, 230036, China.
Laboratory of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, 230036, China.
Plant Physiol Biochem. 2022 Jan 1;170:180-191. doi: 10.1016/j.plaphy.2021.10.024. Epub 2021 Oct 21.
PheWRKY86 is a member of the WRKY transcription factor family in moso bamboo (Phyllostachys edulis). Expression of PheWRKY86 is strongly induced by drought and abscisic acid (ABA) treatments. The PheWRKY86 protein localizes to the cell nucleus and is specifically able to bind to W-box elements. 35S:PheWRKY86 transgenic Arabidopsis and rice showed significantly improved tolerance to drought stress. 35S:PheWRKY86 transgenic plants exhibited better water retention and lower relative electrolyte leakage (REL) and malondialdehyde (MDA) compared to wild type plants. Moreover, 35S:PheWRKY86 transgenic lines showed higher sensitivity to ABA stress. The 35S:PheWRKY86 transgenic plants exhibited higher ABA levels relative to wild type, while also exhibiting a lower germination rate, root length and fresh weight compared to wild type. Further analysis showed that expression of some ABA-responsive genes was changed in the 35S:PheWRKY86 transgenic lines under drought conditions. Transient expression and yeast one-hybrid assays demonstrated that PheWRKY86 could bind to the W-box element in the promoter region of NCED1. Taken together, these results demonstrate that PheWRKY86 plays a positive role in drought tolerance by regulating NCED1 expression.
PheWRKY86 是毛竹（Phyllostachys edulis）WRKY 转录因子家族的一员。PheWRKY86 的表达受干旱和脱落酸（ABA）处理的强烈诱导。PheWRKY86 蛋白定位于细胞核，并且能够特异性地结合 W 框元件。35S:PheWRKY86 转基因拟南芥和水稻表现出对干旱胁迫的显著耐受性提高。与野生型植物相比，35S:PheWRKY86 转基因植物表现出更好的保水能力和更低的相对电解质渗出率（REL）和丙二醛（MDA）。此外，35S:PheWRKY86 转基因系对 ABA 胁迫表现出更高的敏感性。35S:PheWRKY86 转基因植物的 ABA 水平相对野生型升高，而与野生型相比，其发芽率、根长和鲜重降低。进一步的分析表明，在干旱条件下，35S:PheWRKY86 转基因系中一些 ABA 响应基因的表达发生了变化。瞬时表达和酵母单杂交实验表明，PheWRKY86 可以结合 NCED1 启动子区域的 W 框元件。综上所述，这些结果表明 PheWRKY86 通过调节 NCED1 表达在耐旱性中发挥积极作用。
