摩洛哥拉巴特-塞勒-凯尼特拉地区边缘钝缘蜱对二嗪农和甲胺脒敏感性的变化。
Variation of diazinon and amitraz susceptibility of Hyalomma marginatum (Acari: Ixodidae) in the Rabat-Sale-Kenitra region of Morocco.
机构信息
Département de parasitologie et santé publique, Institut Agronomique et Vétérinaire Hassan II, B.P. 6202 Rabat-Instituts, Morocco; Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
出版信息
Ticks Tick Borne Dis. 2022 Mar;13(2):101883. doi: 10.1016/j.ttbdis.2021.101883. Epub 2021 Dec 5.
In the present study, the acaricide susceptibility status of Hyalomma marginatum in Morocco was investigated in the Rabat-Sale-Kenitra region using the Larval Packet Test. The overall LC value for diazinon and amitraz was 115 ppm (95% CI: [104; 125]) and 22 ppm (95% CI: [21; 23]), respectively. The LC values varied significantly between the nine sampled locations (P<0.001) ranging from 75 ppm (95% CI: [65; 84]) in Ouelmes to 179 ppm (95% CI: [139; 201]) in Jorf El Melha for diazinon and from 18 ppm (95% CI: [15; 21]) in Skhirat to 28 ppm (95% CI: [24; 31]) in Ouelmes for amitraz. Sequencing of the target-site of diazinon, acetylcholinesterase 1 (AChE1), indicated that previously reported resistance mutations in AChE1 were absent in ticks from Jorf El Melha surviving 500 ppm diazinon. This study is the first report on the H. marginatum susceptibilty status to the most frequently used acaricides in Morocco and indicates that acaricide tick resistance is emerging.
在本研究中，采用幼虫包被法（Larval Packet Test）调查了摩洛哥拉巴特-塞勒-凯尼特拉地区边缘革蜱（Hyalomma marginatum）的杀蜱剂敏感性状况。二嗪农和双甲脒的 LC 值分别为 115 ppm（95%CI：[104；125]）和 22 ppm（95%CI：[21；23]）。九个采样地点之间的 LC 值差异显著（P<0.001），范围从 75 ppm（95%CI：[65；84]）的 Ouelmes 到 179 ppm（95%CI：[139；201]）的 Jorf El Melha（二嗪农）和 18 ppm（95%CI：[15；21]）的 Skhirat 到 28 ppm（95%CI：[24；31]）的 Ouelmes（双甲脒）。二嗪农靶标位点，乙酰胆碱酯酶 1（AChE1）的测序表明，在 Jorf El Melha 幸存的 500 ppm 二嗪农蜱中不存在先前报道的 AChE1 抗性突变。本研究首次报道了摩洛哥边缘革蜱对最常用杀蜱剂的敏感性状况，表明杀蜱剂蜱抗性正在出现。
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