Reusable smartphone-facilitated mobile fluorescence biosensor for rapid and sensitive on-site quantitative detection of trace pollutants.

Increasing exposure to toxic pollutants highlights the need for their sensitive detection technologies that can be rapidly adapted and deployed in various settings. Optical biosensors are an excellent solution due to their outstanding features. However, the sophisticated and expensive optical design limits their scalability and actual application. Herein, an innovative reusable smartphone-facilitated mobile fluorescence biosensor (s-MFB) was built through integrating miniaturized all-fiber optical system and microfluidic system with smartphone. An asymmetric Y-shaped fiber optic coupler (Y-FOC) is constructed for simultaneous transmission of excitation light and the collected fluorescence. In particular, the incidence rays are introduced into the fiber bio-probe at a specific angle through the single-mode fiber of the Y-FOC, which enhances the evanescent wave field and the number of total internal reflections. The s-MBF showed a LOD for free Cy5.5 of 0.1 nM. Combining indirect competitive immunoassay with the s-MFB, this new assay, which achieve quantitative detection of bisphenol A and norfloxacin in 15 min with high sensitivity and reusability, substantially reduces the complexity and improves the scalability of trace pollutants detection. The adjunctive smartphone application allows on-site real-time quantitative detection, automated interpretation of reporting results, and early-warning of pollution accidents.