Yang Yi, Chen Pei, Liu Yuqing, Cai Zheng, Wang Xiyao, Me Yang, Ding Xiongyu, Lin Lan, Jiang Huijun, Zhang Zhenqin, Ju Yichun
Changzhou Vocational Institute of Engineering, Changzhou 213164, China.
School of Pharmacy, Nanjing Medical University, Nanjing 211166, China; West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Apr 15;251:119479. doi: 10.1016/j.saa.2021.119479. Epub 2021 Jan 18.
Determination of PPi levels in urine represents a measurable factor for diagnostic, treatment, and monitoring of urolithiasis. Owing to the quenching ability of Cu on fluorescent carbon dots (CDs) and strong binding affinity between Cu and PPi, we develop a new off-on assay for PPi detection using newly BPHA CDs (BPHA: N,N-bis(pyridin-2-ylmethyl)hexan-1-amine). The fluorescence intensity of BPHA CDs was significantly quenched by Cu ("off") through forming BPHA CDs/Cu complexes and the fluorescence intensity of BPHA CDs /Cu system was completely resumed by PPi ("on") owing to the release of free Cu. The fluorescence turn-off/on approach showed a highly selective response to PPi over the large family of other anions. The detection limits were 0.094 μM for Cu and 0.025 μM for PPi, respectively. A wide linear range for PPi was up to 4400 μM. The indicator displacement assay (IDAs) using pyrocatechol violet (PV) as a colorimetric indicator was carried out to detect PPi with the naked eyes. The "off-on" fluorescent sensor based on BPHA CDs shows many merits, including convenient operation, cost-saving, high sensitivity, selectivity, stability and wide detecting range, which is applied to PPi detection in human urine sample.
测定尿液中的焦磷酸（PPi）水平是诊断、治疗和监测尿路结石的一个可测量因素。由于铜对荧光碳点（CDs）具有猝灭能力，且铜与PPi之间具有很强的结合亲和力，我们开发了一种新的基于新合成的BPHA CDs（BPHA：N，N-双（吡啶-2-基甲基）己胺-1）的PPi检测开-关分析法。BPHA CDs的荧光强度通过形成BPHA CDs/Cu络合物被铜显著猝灭（“关”），而由于游离铜的释放，BPHA CDs/Cu体系的荧光强度被PPi完全恢复（“开”）。荧光开-关方法对PPi显示出对其他大量阴离子的高度选择性响应。铜和PPi的检测限分别为0.094 μM和0.025 μM。PPi的线性范围高达4400 μM。使用邻苯二酚紫（PV）作为比色指示剂进行了指示剂置换分析（IDAs）以肉眼检测PPi。基于BPHA CDs的“开-关”荧光传感器具有操作方便、成本低、灵敏度高、选择性好、稳定性好和检测范围宽等优点，可应用于人体尿液样本中PPi的检测。
