School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu Province 212013, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province 212013, China.
Colloids Surf B Biointerfaces. 2022 Feb;210:112251. doi: 10.1016/j.colsurfb.2021.112251. Epub 2021 Nov 28.
The aim of the present work is to fabricate immobilized bromelain based on the specific interaction between the cobalt ions of carrier and the inherent cysteines contained in bromelain molecules. The cobalt phosphate material was prepared as solid support by using choline chloride (ChCl)/betaine-glycerol deep eutectic solvent (DES) as solvent and template for the first time. The Co-P material with lamellate-based structure obtained in the ChCl-glycerol DES at the Co/P ratio of 3:2 showed the best performance for the immobilization of bromelain. The specific interaction between Co and bromelain promoted the aggregation of lamellar Co-P, forming flower-like Co-P@bromelain particles. Under the optimum immobilization conditions, the specific enzyme activity of the immobilized enzyme reached the maximum of 71244 U/g. Compared with Co(PO) prepared in water system, the obtained Co-P@bromelain using the Co-P material synthesized in the ChCl-glycerol DES as carrier exhibited excellent structure stability. In addition, the immobilized Co-P@bromelain also showed higher catalytic efficiency than free bromelain.
本工作旨在通过载体钴离子与菠萝蛋白酶分子中固有半胱氨酸之间的特异性相互作用来制备固定化菠萝蛋白酶。首次使用氯化胆碱（ChCl）/甜菜碱-甘油深共晶溶剂（DES）作为溶剂和模板制备磷酸钴材料作为固体支撑物。在 ChCl-甘油 DES 中，当 Co/P 比为 3:2 时，获得了具有层状结构的 Co-P 材料，其对菠萝蛋白酶的固定化性能最佳。Co 与菠萝蛋白酶之间的特异性相互作用促进了层状 Co-P 的聚集，形成了花状 Co-P@菠萝蛋白酶颗粒。在最佳固定化条件下，固定化酶的比酶活达到了 71244 U/g 的最大值。与在水体系中制备的 Co(PO)相比，使用在 ChCl-甘油 DES 中合成的 Co-P 材料作为载体获得的 Co-P@菠萝蛋白酶表现出优异的结构稳定性。此外，固定化 Co-P@菠萝蛋白酶也表现出比游离菠萝蛋白酶更高的催化效率。
