Anal Chem. 2021 Dec 21;93(50):16887-16898. doi: 10.1021/acs.analchem.1c03881. Epub 2021 Dec 12.
Classical chemical probes are prone to dissipation from stressed organelles, as evidenced by the incapability of mitochondrial dyes to image mitophagy linked to multiple diseases. We herein reported mitophagy imaging via ovalent nchoring of a ysosomal probe to the itochondrial inner membrane (). Utilizing , an azide-conjugatable probe with acidity-triggered fluorescence, is operated via ΔΨm-promoted probe accumulation in mitochondria and thereby bioorthogonal ligation of the trapped probe with azido-choline (choline) metabolically installed on the mitochondrial membrane. Overcoming the limitation of synthetic probes to dissipate from stressed organelles, enables signal-on fluorescence imaging of mitophagy induced by starvation and is further employed to reveal mitophagy in ferroptosis. These results suggest the potential of as a new tool to study mitophagy.
经典的化学探针容易从应激细胞器中消散，这可以从线粒体染料无法对与多种疾病相关的自噬体成像中得到证明。在此，我们通过将溶酶体探针共价锚定到线粒体内膜上来报告通过自噬体成像（mitophagy imaging via ovalent nchoring of a ysosomal probe to the itochondrial inner membrane ())。利用一种带有酸度触发荧光的叠氮化物共轭探针，通过 ΔΨm 促进探针在线粒体中的积累，从而在溶酶体探针与代谢安装在线粒体膜上的叠氮胆碱（胆碱）之间进行生物正交连接，来实现这一过程。克服了合成探针从应激细胞器中消散的局限性，使我们能够对饥饿诱导的自噬体进行信号开启荧光成像，并进一步用于揭示铁死亡中的自噬体。这些结果表明，作为一种研究自噬体的新工具，具有很大的潜力。
