Department of Chemical Biology, College of Chemistry and Chemical Engineering, The Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory for Physical Chemistry of Solid Surfaces and The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Xiamen University, Xiamen, Fujian Province 361005, China.
State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian Province 361005, China.
Anal Chem. 2020 Nov 17;92(22):15059-15068. doi: 10.1021/acs.analchem.0c03029. Epub 2020 Nov 3.
Metabolic glycan labeling (MGL) has been employed for diverse purposes, such as cell surface glycan imaging and tumor surface engineering. We herein reported organelle-specific MGL (OMGL) for selective tagging of the inner limiting membrane of lysosomes over the cell surface. This is operated via acidity-promoted accumulation of optical probes in lysosomes and bioorthogonal ligation of the trapped probes with 9-azidosialic acid (Sia) metabolically installed on lysosomal membrane proteins. Overcoming the limitation of classical organelle probes to dissipate from stressed organelles, OMGL enables optical tracking of pH-elevated lysosomes in exocytosis and membrane-permeabilized lysosomes in different cell death pathways. Thus, OMGL offers a new tool to study lysosome biology.
代谢糖基化标记（MGL）已经被应用于多种目的，例如细胞表面糖基化成像和肿瘤表面工程。我们在此报告细胞器特异性 MGL（OMGL），用于选择性标记溶酶体的内限膜，而不是细胞表面。这是通过在溶酶体中促进酸性积累光学探针，并通过生物正交连接与溶酶体膜蛋白上代谢安装的 9-叠氮唾液酸（Sia）捕获的探针来实现的。克服了经典细胞器探针从应激细胞器中消散的限制，OMGL 能够光学跟踪细胞外泌体中 pH 值升高的溶酶体和不同细胞死亡途径中膜通透的溶酶体。因此，OMGL 为研究溶酶体生物学提供了一种新工具。
