将贝司他汀和硫磷酰胺注射到兔伏隔核可增强电针和吗啡镇痛作用。
Electroacupuncture and morphine analgesia potentiated by bestatin and thiorphan administered to the nucleus accumbens of the rabbit.
作者信息
Jin W Q, Zhou Z F, Han J S
出版信息
Brain Res. 1986 Aug 20;380(2):317-24. doi: 10.1016/0006-8993(86)90228-3.
The endogenous opioid peptide enkephalin (EK) is known to be degraded mainly by two enzymes, the dipeptidyl carboxypeptidase 'enkephalinase' and aminopeptidase. Microinjection of the enkephalinase inhibitor thiorphan or the aminopeptidase inhibitor bestatin into the nucleus accumbens of the rabbit produced a dose-dependent analgesic effect. This analgesic effect was totally reversed by the narcotic antagonist naloxone or by antibodies against [Met5]enkephalin (MEK) administered to the same site. Antibodies against [Leu5]enkephalin were not effective. Moreover, microinjection of thiorphan or bestatin into the nucleus accumbens resulted in a marked potentiation of the aftereffect of electroacupuncture (EA) produced analgesia, as well as the analgesia induced by a small dose of morphine. It is concluded that the analgesic effect elicited by EA and morphine is mediated, at least in part, by MEK-like immunoreactive substance(s) in the nucleus accumbens.
已知内源性阿片肽脑啡肽（EK）主要由两种酶降解，即二肽基羧肽酶“脑啡肽酶”和氨肽酶。将脑啡肽酶抑制剂硫磷酰胺或氨肽酶抑制剂贝司他汀微量注射到兔伏隔核中会产生剂量依赖性镇痛作用。这种镇痛作用可被麻醉拮抗剂纳洛酮或在同一部位注射的抗[Met5]脑啡肽（MEK）抗体完全逆转。抗[Leu5]脑啡肽抗体无效。此外，将硫磷酰胺或贝司他汀微量注射到伏隔核中会导致电针（EA）产生的镇痛后效应以及小剂量吗啡诱导的镇痛显著增强。得出的结论是，EA和吗啡引发的镇痛作用至少部分是由伏隔核中类似MEK的免疫反应性物质介导的。
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