Department of Pharmaceutical Biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Pharmaceutical Biotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Pharm Dev Technol. 2022 Jan;27(1):19-24. doi: 10.1080/10837450.2021.2018457. Epub 2021 Dec 21.
In this project, a biocompatible block copolymer including poly ethylene glycol and poly caprolactone was synthesized using ring-opening reaction. Then, the copolymer was conjugated to folic acid using lysine as a linker. Also, curcumin (CUR) was used as a therapeutic anticancer agent. Nanoprecipitation method was used to prepare CUR-loaded polymeric micelles. Different methods including Fourier-transform infrared spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS) were used to characterize the prepared nanocarriers (NCs). MTT assay and hemolysis assay were used to evaluate anticancer efficiency and biocompatibility of the prepared NCs, respectively. The results proved efficiency of NCs as a drug delivery system (DDS) in various aspects such as physicochemical properties and biocompatibility. Also, results showed that NCs did not show any severe weight loss and side effects on mice, and the anti-cancer study results of the CUR-loaded NCs proved that the conjugation of folic acid on the surface of NCs as a targeting agent could increase the therapeutic efficacy of CUR.
在这个项目中，使用开环反应合成了一种包括聚乙二醇和聚己内酯的生物相容性嵌段共聚物。然后，通过赖氨酸作为连接物将共聚物与叶酸偶联。同时，姜黄素（CUR）被用作治疗性抗癌药物。使用纳米沉淀法制备载 CUR 的聚合物胶束。使用傅里叶变换红外光谱、透射电子显微镜（TEM）和动态光散射（DLS）等不同方法对制备的纳米载体（NCs）进行了表征。MTT 测定和溶血试验分别用于评估制备的 NCs 的抗癌效率和生物相容性。结果证明了 NCs 作为药物传递系统（DDS）在物理化学性质和生物相容性等各个方面的有效性。此外，结果表明 NCs 对小鼠没有任何严重的体重减轻和副作用，载 CUR 的 NCs 的抗癌研究结果证明，将叶酸偶联到 NCs 表面作为靶向剂可以提高 CUR 的治疗效果。
