Saito K, Adler L T
Cell Immunol. 1986 Apr 15;99(1):209-19. doi: 10.1016/0008-8749(86)90229-7.
The question of the ultimate fate of lymphocytes subjected to treatment with anti-allotype antibody (Ab) has been investigated by means of an adoptive transfer system that uses noninbred rabbits matched for major histocompatibility antigens and mismatched for allotype. Suppression of b4 immunoglobulin (Ig) production was induced by incubating lymphocytes from b4b5 rabbits with anti-b4 in culture. Transfer of b4-suppressed cells to newborn recipients of allotype b6b6 resulted in stable chimerism of mixed donor-recipient allotypes, in which b4 Ig production remained suppressed. In recipients of non-Ab-treated cells, b4 Ig production predominated over b5, as had been the case in the intact donor. No evidence for stimulation of b4 Ig synthesis was seen, even when lymphocytes and serum from 1-week-old recipients were examined. When lymphoid cells of antigen-primed b4b5 donors were treated with anti-b4 in vitro and transferred, Ab production of the b4 type was specifically suppressed, with compensatory over-production by Ab-forming cells of the b5 type. The results reported here indicate that although anti-allotype Ab is not directly cytotoxic, a significant proportion of the b4-committed cells were irreversibly inactivated as a result of Ab pulse treatment.
通过一种采用主要组织相容性抗原匹配且同种异型不匹配的非近交系兔子的过继转移系统，研究了用抗同种异型抗体（Ab）处理的淋巴细胞的最终命运问题。通过在培养中将来自b4b5兔子的淋巴细胞与抗b4一起孵育，诱导b4免疫球蛋白（Ig）产生的抑制。将b4抑制的细胞转移到同种异型为b6b6的新生受体中，导致混合供体-受体同种异型的稳定嵌合体，其中b4 Ig的产生仍然受到抑制。在未用Ab处理的细胞的受体中，b4 Ig的产生比b5占优势，就像在完整供体中一样。即使检查1周龄受体的淋巴细胞和血清，也没有发现刺激b4 Ig合成的证据。当用抗b4体外处理并转移经抗原致敏的b4b5供体的淋巴细胞时，b4型Ab的产生被特异性抑制，b5型Ab形成细胞代偿性过度产生。此处报道的结果表明，尽管抗同种异型Ab没有直接细胞毒性，但由于Ab脉冲处理，相当一部分b4定向细胞被不可逆地灭活。
