Roitman Karina L, Almuzara Marisa N, Fernández Rodriguez Mario Javier, Basbus Federico, Cipolla Lucía, Barberis Claudia M, Vay Carlos A
Instituto Nacional de Enfermedades Infecciosas (INEI)-Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) Dr. Carlos G. Malbrán, Ciudad Autónoma de Buenos Aires, Argentina.
Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Universidad de Buenos Aires, Buenos Aires, Argentina; Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Fisiopatología y Bioquímica Clínica INFIBIOC, Buenos Aires, Argentina.
Rev Argent Microbiol. 2022 Apr-Jun;54(2):106-109. doi: 10.1016/j.ram.2021.10.002. Epub 2021 Dec 8.
Dolosigranulum pigrum is a gram-positive, facultatively anaerobic coccus, which is part of the oral and upper respiratory tract microbiota. Although reports of infections by this microorganism are scarce, it has been associated with a wide spectrum of infectious diseases. The case of an elderly man with a lower corneal abscess, in which Dolosigranulum pigrum was isolated, is described. The microorganism was identified by mass spectrometry (MALDI-TOF MS) and by the sequencing of the 16S rRNA gene. Furthermore, the presumptive identification of the causative agent was achieved by using key phenotypic tests such as the cluster arrangement in Gram stain, the negative catalase test, the production of pyrrolidonyl arylamidase and leucine aminopeptidase activity, the growth in 6.5% NaCl and esculin hydrolysis. The data from the literature (and the present case) support the association of the microorganism with ocular infections, which often take a destructive course, mainly in elderly patients.
惰性多尔氏菌是一种革兰氏阳性兼性厌氧球菌，是口腔和上呼吸道微生物群的一部分。尽管关于这种微生物感染的报道很少，但它与多种传染病有关。本文描述了一例老年男性角膜下脓肿病例，从中分离出了惰性多尔氏菌。该微生物通过质谱分析（基质辅助激光解吸电离飞行时间质谱，MALDI-TOF MS）和16S rRNA基因测序进行鉴定。此外，通过革兰氏染色中的菌簇排列、过氧化氢酶阴性试验、吡咯烷酮芳基酰胺酶的产生和亮氨酸氨肽酶活性、在6.5%氯化钠中的生长以及七叶苷水解等关键表型试验，实现了对病原体的初步鉴定。文献数据（以及本病例）支持该微生物与眼部感染的关联，眼部感染通常呈破坏性病程，主要发生在老年患者中。
