Zharhary D
Eur J Immunol. 1986 Sep;16(9):1175-8. doi: 10.1002/eji.1830160924.
The role of T cells in the reduced frequency of splenic B cells specific for several antigens in aged mice was studied by assessing B cell responsiveness in (a) aged nude mice and (b) irradiated young mice repopulated with splenic B cells or with Ig- bone marrow cells from young mice and T cells from aged vs. young mice. Using the fragment culture technique to assess B cells specific for 2,4-dinitrophenyl (DNP) and for (4-hydroxy-3,5-dinitrophenyl) acetyl, we found that the frequency of responsive splenic B cells in aged BALB/c nude mice was very similar to that of young nude mice. In addition, we found that in chimeric mice constructed with either bone marrow or splenic B cells from young mice and T cells from aged mice the frequency of DNP-specific splenic B cells was significantly lower than that in control chimeras constructed with T cells from young mice. These results indicate that T cells from aged mice can down regulate B cell responsiveness and that a mature, naive B cell may be its possible target. The results of both experimental approaches are consistent with a role for T cells in the regulation of responsive B cells in aging.
通过评估以下两种情况的B细胞反应性，研究了T细胞在老年小鼠中针对几种抗原的脾脏B细胞频率降低中的作用：（a）老年裸鼠；（b）用来自年轻小鼠的脾脏B细胞或Ig-骨髓细胞以及来自老年与年轻小鼠的T细胞重新填充的经辐射的年轻小鼠。使用片段培养技术评估对2,4-二硝基苯基（DNP）和（4-羟基-3,5-二硝基苯基）乙酰基有特异性的B细胞，我们发现老年BALB/c裸鼠中反应性脾脏B细胞的频率与年轻裸鼠非常相似。此外，我们发现，在用来自年轻小鼠的骨髓或脾脏B细胞以及来自老年小鼠的T细胞构建的嵌合小鼠中，DNP特异性脾脏B细胞的频率显著低于用来自年轻小鼠的T细胞构建的对照嵌合体。这些结果表明，来自老年小鼠的T细胞可以下调B细胞反应性，并且成熟的幼稚B细胞可能是其潜在靶点。两种实验方法的结果均与T细胞在衰老过程中调节反应性B细胞的作用一致。
