Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, PR China.
Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, PR China; Taiyuan University of Technology, Taiyuan, Shanxi 030024, PR China.
J Hazard Mater. 2022 Mar 5;425:127947. doi: 10.1016/j.jhazmat.2021.127947. Epub 2021 Dec 1.
Cadmium (Cd) is a harmful heavy metal that affects the growth and development of plants. Nitrogen (N) is an essential nutrient for plants, and appropriate N management can improve Cd tolerance. The aim of our study was to explore the effects of different forms of N on the molecular and physiological responses of the hyperaccumulator Solanum nigrum to Cd toxicity. Measurement of biomass, photosynthetic parameters, and Cd fluxes using non-invasive micro-test technique, Cd fluorescent dying, biochemical methods and quantitative real-time PCR analysis were performed in our study. Our results showed that ammonium (NH) has stronger Cd detoxification ability than nitrate (NO), which are likely attributed to the following three reasons: (1) NH decreased the influx and accumulation of Cd by regulating the transcription of Cd transport-related genes; (2) the ameliorative effects of NH were accompanied by the increased retention of Cd in the cell walls of roots; and (3) NH up-regulated SnExp expression.
镉（Cd）是一种有害的重金属，会影响植物的生长和发育。氮（N）是植物的必需营养元素，适当的 N 管理可以提高 Cd 耐受性。本研究旨在探讨不同形式的 N 对超积累植物龙葵对 Cd 毒性的分子和生理响应的影响。我们使用非侵入性微测技术、Cd 荧光染色、生化方法和定量实时 PCR 分析来测量生物量、光合参数和 Cd 通量。我们的结果表明，铵（NH）比硝酸盐（NO）具有更强的 Cd 解毒能力，这可能归因于以下三个原因：（1）NH 通过调节 Cd 转运相关基因的转录来减少 Cd 的内流和积累；（2）NH 的改善作用伴随着 Cd 在根系细胞壁中保留的增加；（3）NH 上调了 SnExp 的表达。
