Truman P, Ford H C
In Vitro Cell Dev Biol. 1986 Sep;22(9):525-8. doi: 10.1007/BF02621138.
Attempts were made to select for trophoblast cells in cultures of mixed cell populations derived from preterm (7 to 12 wk) or term human placentas. Epidermal growth factor added to cultures on solid or porous supports caused proliferation of epithelial-type cells to give a confluent monolayer but did not increase the expression of differentiated function. The presence or absence of placental basement membrane collagen as substrate made little apparent difference; however a porous basement membrane collagen support led to increased differentiated function. Initial production of human chorionic gonadotrophin was increased and after 4 wk in culture a substantial proportion of the cells exhibited alkaline phosphatase activity. Epidermal growth factor and a substrate of placental basement membrane collagen on a porous support favorably influence the growth and differentiation of human trophoblast cells in culture.
人们尝试从早产（7至12周）或足月的人胎盘来源的混合细胞群体培养物中筛选滋养层细胞。添加到固体或多孔支持物上的培养物中的表皮生长因子会导致上皮型细胞增殖，形成汇合的单层，但不会增加分化功能的表达。作为底物的胎盘基底膜胶原蛋白的存在与否似乎没有明显差异；然而，多孔基底膜胶原蛋白支持物会导致分化功能增强。人绒毛膜促性腺激素的初始产量增加，培养4周后，相当一部分细胞表现出碱性磷酸酶活性。表皮生长因子和多孔支持物上的胎盘基底膜胶原蛋白底物对培养的人滋养层细胞的生长和分化有有利影响。
