Choi Cheol-Woo, Jung Kyung-Eun, Kim Min-Ju, Yoon Su-Hyeon, Park Suk-Man, Jin Seong-Beom, Hyun Jae-Wook
Citrus Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration, Jeju 63607, Korea.
Plant Pathol J. 2021 Dec;37(6):681-686. doi: 10.5423/PPJ.NT.07.2021.0117. Epub 2021 Dec 1.
It is difficult to distinguish melanose and melanoses-like symptoms with the naked eye because they appear similar. To accurately detect melanose symptoms caused by Diaporthe citri from melanose-like symptoms, we developed PCR-based specific primers Dcitri by aligning the internal transcribed spacer (ITS) region of D. citri with the ITS of Diaporthe cytosporella, Diaporthe foeniculina, Colletotrichum gloeosporioides, Botrytis cinerea, Alternaria citri, and Fusarium oxysporum found on citrus peel. PCR results showed that the specific product was amplified in D. citri but not in other isolates including, C. gloeosporioides, B. cinerea, A. citri, F. oxysporum. In addition, specific products were observed in melanose symptoms caused by D. citri but not in melanose-like symptoms, such as copper-injury, sunscald, damages by yellow tea thrips, and pink citrus rust mite. Using the Dcitri primers developed in this study, it is expected that melanose caused by D. citri could be accurately distinguished from melanose-like symptoms.
肉眼很难区分黑星病和类似黑星病的症状，因为它们看起来很相似。为了从类似黑星病的症状中准确检测出由柑桔间座壳菌引起的黑星病症状，我们通过将柑桔间座壳菌的内转录间隔区（ITS）区域与在柑橘果皮上发现的拟茎点霉、茴香拟茎点霉、胶孢炭疽菌、灰葡萄孢、柑橘链格孢和尖孢镰刀菌的ITS进行比对，开发了基于PCR的特异性引物Dcitri。PCR结果表明，在柑桔间座壳菌中扩增出了特异性产物，而在包括胶孢炭疽菌、灰葡萄孢、柑橘链格孢、尖孢镰刀菌在内的其他分离物中未扩增出。此外，在由柑桔间座壳菌引起的黑星病症状中观察到了特异性产物，而在类似黑星病的症状中未观察到，如铜害、日灼、黄蓟马危害和柑橘锈壁虱危害。使用本研究中开发的Dcitri引物，有望将柑桔间座壳菌引起的黑星病与类似黑星病的症状准确区分开来。
