Department of Medical Neuroscience, and Brain Repair Centre, Faculty of Medicine, Dalhousie University, Life Science Research Institute, Halifax, Nova Scotia, Canada.
Genesis. 2022 Feb;60(1-2):e23464. doi: 10.1002/dvg.23464. Epub 2021 Dec 13.
Brain acid soluble protein 1 (BASP1; previously NAP22 or CAP23) is an actin-associating protein that is highly expressed in the nervous system throughout development. While its roles at the neuromuscular junction and in certain non-neuronal tissues have been previously characterized, its function in the early neural tube is unclear. Using in ovo electroporation in the chicken (Gallus gallus) embryonic neural tube, we show that BASP1 overexpression resulted in the appearance of ectopic neural progenitor cells within the marginal zone of the neural tube. BASP1 knockdown did not affect the position of neural progenitors but did alter the complexity of axons developing from differentiated neurons. This suggests a role for BASP1 in regulating the apical polarity of progenitor cells and axon trajectories from developing neurons.
脑酸溶性蛋白 1（BASP1；以前称为 NAP22 或 CAP23）是一种与肌动蛋白结合的蛋白，在神经系统发育过程中高度表达。虽然它在神经肌肉接头和某些非神经元组织中的作用以前已经得到了描述，但它在早期神经管中的功能尚不清楚。我们使用鸡（Gallus gallus）胚胎神经管中的胚胎电穿孔，表明 BASP1 的过表达导致神经管边缘区出现异位神经祖细胞。BASP1 的敲低并不影响神经祖细胞的位置，但确实改变了分化神经元发育的轴突的复杂性。这表明 BASP1 在调节祖细胞的顶端极性和发育神经元的轴突轨迹方面发挥作用。
