Ying Yong Sheng Tai Xue Bao. 2021 Nov 15;32(11):4095-4106. doi: 10.13287/j.1001-9332.202111.033.
Soil samples were collected at the fallow period, flowering stage, and fruiting stage of tomato under tomato-melon (TM) and tomato-bean (TB) systems. Illumina MiSeq high-throughput pyrosequencing was performed to analyze the differences in AM fungal community between the two rotation systems. We further analyzed the key factors driving the changes in AM fungal diversity and community composition. Results showed that rotation with legume significantly altered the α-diversity of AM fungi. Shannon diversity and Pielou evenness of AM fungi under the TB system were 24.9% and 24.0% lower than that under TM system, respectively. Compared to the fallow period, richness, Shannon diversity, and phylogenetic diversity of AM fungi at the tomato flowering and fruiting stages decreased significantly by 55.6%-67.5%, 49.6%-51.5%, and 21.4%-23.7%, respectively. Rotation with legume (the TB system) promoted the relative abundance of Glomus in all the three sampling times, but reduced the relative abundance of Paraglomus and Archaeospora at the flowering and fruiting stages. Claroideoglomus was more abundant in soils under the TM system than that under the TB system at the fallow period, but the pattern was the opposite at the flowering stage. Ambispora, Diversispora, and Scutellospora were detected only in soil under the TB system. Results of permutational multivariate analysis of variance (PERMANOVA) and non-metric multidimensional scaling (NMDS) analysis showed that both rotation system and growing stage significantly affected the structure of AM fungal community. Soil moisture, pH, and Olsen-P were the predominant factors controlling the variations in the diversity and composition of AM fungal community. Results of structural equation modeling (SEM) further indicated that rotation system and growing stage affected the variations in AM fungal diversity and community structure indirectly via changing soil pH.
在番茄-瓜（TM）和番茄-豆（TB）系统下，分别在番茄休耕期、花期和结果期采集土壤样本。使用 Illumina MiSeq 高通量焦磷酸测序分析两种轮作系统中 AM 真菌群落的差异。我们进一步分析了驱动 AM 真菌多样性和群落组成变化的关键因素。结果表明，豆科植物轮作显著改变了 AM 真菌的α多样性。TB 系统下 AM 真菌的 Shannon 多样性和 Pielou 均匀度分别比 TM 系统低 24.9%和 24.0%。与休耕期相比，番茄开花期和结果期 AM 真菌的丰富度、Shannon 多样性和系统发育多样性分别显著下降了 55.6%-67.5%、49.6%-51.5%和 21.4%-23.7%。豆科植物轮作（TB 系统）促进了所有三个采样时间内 Glomus 的相对丰度，但降低了开花期和结果期 Paraglomus 和 Archaeospora 的相对丰度。Claroideoglomus 在 TM 系统下的土壤中比在 TB 系统下的土壤中更为丰富，而在休耕期则相反。在 TB 系统下的土壤中检测到 Ambispora、Diversispora 和 Scutellospora，而在 TM 系统下则未检测到。PERMANOVA 和 NMDS 分析的结果表明，轮作系统和生长阶段显著影响 AM 真菌群落的结构。土壤水分、pH 值和 Olsen-P 是控制 AM 真菌群落多样性和组成变化的主要因素。结构方程模型（SEM）的结果进一步表明，轮作系统和生长阶段通过改变土壤 pH 值间接影响 AM 真菌多样性和群落结构的变化。
