Allavena P, Zanaboni F, Rossini S, Merendino A, Bonazzi C, Vassena L, Mangioni C, Mantovani A
J Natl Cancer Inst. 1986 Oct;77(4):863-8.
Peripheral blood lymphocytes (PBLs) and tumor-associated lymphocytes (TALs) were isolated from 36 patients with advanced ovarian adenocarcinoma and peritoneal effusions for study of lymphokine-activated killer activity. PBLs and TALs cultured in vitro for 3-5 days in the presence of interleukin-2 (IL-2, supernatant of the MLA 144 gibbon cell line, or human recombinant IL-2) expressed higher levels of cytotoxicity as compared to cells cultured in medium alone, against natural killer (NK)-susceptible (K562) or NK-resistant targets (Daudi and the human ovarian carcinoma cell line SW626). When ovarian tumor cells, freshly isolated from carcinomatous ascites or surgical specimens, were used as target cells in the cytotoxicity assay, 8 of 14 PBLs and 5 of 7 TAL preparations lysed the autologous tumor after treatment with IL-2, while no spontaneous reactivity was observed in any of the 14 patients tested. Although levels of lysis were usually relatively low, these data demonstrate that PBLs and TALs from ovarian cancer patients (TALs usually exhibiting low NK activity) when stimulated in vitro by IL-2 acquire some cytotoxic potential against the autologous tumor.
从36例晚期卵巢腺癌伴腹水患者中分离出外周血淋巴细胞（PBL）和肿瘤相关淋巴细胞（TAL），用于研究淋巴因子激活的杀伤活性。与仅在培养基中培养的细胞相比，PBL和TAL在白细胞介素-2（IL-2，长臂猿MLA 144细胞系的上清液或人重组IL-2）存在的情况下体外培养3-5天，对自然杀伤（NK）敏感靶细胞（K562）或NK抗性靶细胞（Daudi和人卵巢癌细胞系SW626）表现出更高的细胞毒性水平。当从癌性腹水或手术标本中新鲜分离的卵巢肿瘤细胞用作细胞毒性试验的靶细胞时，14份PBL制剂中的8份和7份TAL制剂中的5份在用IL-2处理后裂解了自体肿瘤，而在测试的14例患者中均未观察到自发反应性。尽管裂解水平通常相对较低，但这些数据表明，卵巢癌患者的PBL和TAL（TAL通常表现出低NK活性）在体外受到IL-2刺激后获得了对自体肿瘤的一定细胞毒性潜力。
