Katayama Masafumi, Onuma Manabu, Fukuda Tomokazu
Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, 16-2, Onogawa, Tsukuba, Ibaraki, 305-8506, Japan.
Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies, 16-2, Onogawa, Tsukuba, Ibaraki, 305-8506, Japan.
J Poult Sci. 2021 Oct 25;58(4):270-279. doi: 10.2141/jpsa.0200085.
Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco's modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists.
培养细胞对家禽科学家来说是一种有用的资源，因为这些细胞使科学家能够在模拟鸟类全身反应的同时，评估对诸如传染病等条件的生物学反应。然而，禽类细胞培养需要优化的基础培养基，而目前这种基础培养基的选择相对较少（199培养基和KAv-1）。这意味着开发一种用于禽类细胞培养的最佳基础培养基仍有空间。在这里，我们在鸡成纤维细胞培养过程中比较了KAv-1培养基、杜氏改良 Eagle 培养基（DMEM）和199培养基，并确定KAv-1仍然是这些试验的最佳培养基。我们的结果表明，与DMEM和199培养基相比，在KAv-1培养基中培养的成纤维细胞DNA损伤减少，并且与DMEM和199培养基相比，这些细胞在KAv-1培养基中也表现出更好的生长动力学。据我们所知，这是第一项描述禽类细胞培养基比较分析的研究，这将为家禽科学家提供有用的信息。
