Wang Xiang-Xu, Wu Li-Hong, Ai Liping, Pan Wei, Ren Jing-Yi, Zhang Qiong, Zhang Hong-Mei
Department of Clinical Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
Xijing 986 Hospital Department, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
Mol Ther Nucleic Acids. 2021 Nov 10;26:1387-1400. doi: 10.1016/j.omtn.2021.11.006. eCollection 2021 Dec 3.
Long noncoding RNAs (lncRNAs) are emerging as critical regulators of gene expression and play fundamental roles in immune regulation. Growing evidence suggests that immune-related genes and lncRNAs can serve as markers to predict the prognosis of patients with cancers, including hepatocellular carcinoma (HCC). This study aimed to contract an immune-related lncRNA (IR-lncRNA) signature for prospective assessment to predict early recurrence of HCC. A total of 319 HCC samples under radical resection were randomly divided into a training cohort (161 samples) and a testing cohort (158 samples). In the training dataset, univariate, lasso, and multivariate Cox regression analyses identified a 9-IR-lncRNA signature closely related to disease-free survival. Kaplan-Meier analysis, principal component analysis, gene set enrichment analysis, and nomogram were used to evaluate the risk model. The results were further confirmed in the testing cohort. Furthermore, we constructed a competitive endogenous RNA regulatory network. The results of the present study indicated that this 9-IR-lncRNA signature has important clinical implications for improving predictive outcomes and guiding individualized treatment in HCC patients. These IR-lncRNAs and regulated genes may be potential biomarkers associated with the prognosis of HCC.
长链非编码RNA（lncRNAs）正逐渐成为基因表达的关键调节因子，并在免疫调节中发挥着重要作用。越来越多的证据表明，免疫相关基因和lncRNAs可作为预测包括肝细胞癌（HCC）在内的癌症患者预后的标志物。本研究旨在构建一种免疫相关lncRNA（IR-lncRNA）特征，用于前瞻性评估以预测HCC的早期复发。总共319例接受根治性切除的HCC样本被随机分为训练队列（161例样本）和测试队列（158例样本）。在训练数据集中，单因素、套索和多因素Cox回归分析确定了一个与无病生存期密切相关的9-IR-lncRNA特征。采用Kaplan-Meier分析、主成分分析、基因集富集分析和列线图来评估风险模型。结果在测试队列中得到进一步证实。此外，我们构建了一个竞争性内源性RNA调控网络。本研究结果表明，这种9-IR-lncRNA特征对于改善HCC患者的预测结果和指导个体化治疗具有重要的临床意义。这些IR-lncRNAs及其调控的基因可能是与HCC预后相关的潜在生物标志物。
