Gutiérrez Jesús Alfredo Berdugo, Cardona Maya Walter D
- Reproduction Group, Department of Microbiology and Parasitology, Medical School, University of Antioquia, Antioquia, Colombia.
J Reprod Infertil. 2021 Jul-Sep;22(3):210-215. doi: 10.18502/jri.v22i3.6721.
Male infertility is associated with altered characteristics of the sperm within the ejaculate. It is possible to find molecular explanations for the observed phenotypes and their consequences. This study aimed to analyze, using a specialized software, a gene set of transcriptomic data from different types of ejaculates.
Data from ejaculate samples categorized as normal, oligospermia, and teratozoospermia were obtained from Gene Expression Omnibus (GEO). After normalization, the data average for each sample category was calculated and analyzed independently using Ingenuity Pathway Analysis (IPA).
Five important canonical pathways are involved in normal and altered semen samples (Oligospermia and teratozoospermia) except sirtuin signaling and mitochondrial dysfunction pathways. The five most important biological processes are identified in all semen phenotypes, but the only difference is the genes connected with initiation of RNA transcription in oligospermic and asthenospermic samples.
Surprisingly, different types of ejaculates share many pathways and biological processes; sperm proteomics as a new global approach gives clues for the development of strategies to explain the reason for observed phenotypes of ejaculated spermatozoa, their possible effect on fertility, and for implementing research strategies in the context of infertility diagnosis and treatment.
男性不育与射精精液中精子特征的改变有关。有可能找到所观察到的表型及其后果的分子解释。本研究旨在使用专门软件分析来自不同类型射精精液的转录组数据基因集。
从基因表达综合数据库（GEO）获取分类为正常、少精子症和畸形精子症的射精精液样本数据。标准化后，计算每个样本类别的数据平均值，并使用 Ingenuity 通路分析（IPA）独立进行分析。
除了沉默调节蛋白信号传导和线粒体功能障碍通路外，正常和异常精液样本（少精子症和畸形精子症）涉及五条重要的经典通路。在所有精液表型中都鉴定出了五个最重要的生物学过程，但唯一的区别是少精子症和弱精子症样本中与 RNA 转录起始相关的基因。
令人惊讶的是，不同类型的射精精液共享许多通路和生物学过程；精子蛋白质组学作为一种新的全局方法为制定策略提供了线索，以解释射精精子所观察到的表型的原因、它们对生育能力的可能影响，以及在不育诊断和治疗背景下实施研究策略。
