Zhang Kunli, Chu Pinpin, Song Shuai, Yang Dongxia, Bian Zhibiao, Li Yan, Gou Hongchao, Jiang Zhiyong, Cai Rujian, Li Chunling
Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, China.
Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China.
Front Vet Sci. 2021 Nov 26;8:756764. doi: 10.3389/fvets.2021.756764. eCollection 2021.
has emerged as an important bacterial pathogen in pig husbandry, as can coinfect pigs with a variety of pathogenic microorganisms and further cause an aggravation of the disease. It is crucial to investigate its pathogenetic mechanism. Gram-negative bacteria naturally secrete outer membrane vesicles (OMVs), and their potent virulence factors play prominent roles that affect the interaction between bacteria and host. Still, the pathogenesis that is associated with the bacterial OMVs has not been well-elucidated. In this study, we investigated the secretion of OMVs from a clinical isolate strain (H45). In addition, we further analyzed the characterization, the comprehensive proteome, and the virulence potential of OMVs. Our data demonstrated that could secrete OMVs into the extracellular milieu during infection. Using liquid chromatography with tandem mass spectrometry (MS/MS) identification and bio-information analysis, we identified 588 different proteins associated with OMVs. Also, we also analyzed the subcellular location and biological function of those proteins. These proteins are mainly involved in immune and iron metabolism. Moreover, we confirmed the pathogenicity of OMVs by observing a strong inflammatory response in J774A.1 and porcine alveolar macrophages. Taken together, our findings suggested that OMVs from were involved in the pathogenesis of this bacterium during infection.
已成为养猪业中一种重要的细菌病原体，因为它可与多种致病微生物共同感染猪，并进一步导致疾病加重。研究其致病机制至关重要。革兰氏阴性菌自然分泌外膜囊泡（OMVs），其强大的毒力因子在影响细菌与宿主相互作用方面发挥着重要作用。然而，与细菌OMVs相关的发病机制尚未得到充分阐明。在本研究中，我们调查了临床分离株（H45）的OMVs分泌情况。此外，我们进一步分析了OMVs的特性、综合蛋白质组和毒力潜力。我们的数据表明，在感染过程中可将OMVs分泌到细胞外环境中。通过液相色谱串联质谱（MS/MS）鉴定和生物信息分析，我们鉴定出588种与OMVs相关的不同蛋白质。此外，我们还分析了这些蛋白质的亚细胞定位和生物学功能。这些蛋白质主要参与免疫和铁代谢。此外，我们通过观察J774A.1和猪肺泡巨噬细胞中的强烈炎症反应，证实了OMVs的致病性。综上所述，我们的研究结果表明，来自该菌的OMVs在感染过程中参与了该细菌的发病机制。
