Wang Lulu, Zhang Xiaokang, Xu Pingping, Yan Jicheng, Zhang Yuzhong, Su Hainan, Sun Chengjun, Lu Qiang, Liu Weizhi
Sars-Fang Centre, MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, 266071, China.
Bioact Mater. 2021 Sep 4;10:504-514. doi: 10.1016/j.bioactmat.2021.08.021. eCollection 2022 Apr.
Antioxidant biomaterials have attracted much attention in various biomedical fields because of their effective inhibition and elimination of reactive oxygen species (ROS) in pathological tissues. However, the difficulty in ensuring biocompatibility, biodegradability and bioavailability of antioxidant materials has limited their further development. Novel bioavailable antioxidant materials that are derived from natural resources are urgently needed. Here, an integrated multi-omics method was applied to fabricate antioxidant biomaterials. A key cysteine-rich thrombospondin-1 type I repeat-like (TSRL) protein was efficiently discovered from among 1262 adhesive components and then used to create a recombinant protein with a yield of 500 mg L. The biocompatible TSRL protein was able to self-assemble into either a water-resistant coating through Ca-mediated coordination or redox-responsive hydrogels with tunable physical properties. The TSRL-based hydrogels showed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rates than glutathione (GSH) and ascorbic acid (Aa) and protected cells against external oxidative stress significantly more effectively. When topically applied to mice skin, TSRL alleviated epidermal hyperplasia and suppressed the degradation of collagen and elastic fibers caused by ultraviolet radiation B (UVB) irradiation, confirming that it enhanced antioxidant activity . This is the first study to successfully characterize natural antioxidant biomaterials created from marine invertebrate adhesives, and the findings indicate the excellent prospects of these biomaterials for great applications in tissue regeneration and cosmeceuticals.
抗氧化生物材料因其能有效抑制和清除病理组织中的活性氧(ROS)而在各种生物医学领域备受关注。然而,确保抗氧化材料的生物相容性、生物降解性和生物利用度存在困难,这限制了它们的进一步发展。迫切需要源自自然资源的新型生物可利用抗氧化材料。在此,应用了一种综合多组学方法来制备抗氧化生物材料。从1262种粘附成分中高效发现了一种关键的富含半胱氨酸的血小板反应蛋白1型I重复样(TSRL)蛋白,然后用于制备产量为500 mg/L的重组蛋白。具有生物相容性的TSRL蛋白能够通过钙介导的配位自组装成防水涂层,或形成具有可调物理性质的氧化还原响应水凝胶。基于TSRL的水凝胶显示出比谷胱甘肽(GSH)和抗坏血酸(Aa)更强的1,1-二苯基-2-苦基肼(DPPH)自由基清除率,并且能更有效地保护细胞免受外部氧化应激。当局部应用于小鼠皮肤时,TSRL减轻了表皮增生,并抑制了紫外线B(UVB)照射引起的胶原蛋白和弹性纤维降解,证实其增强了抗氧化活性。这是首次成功表征由海洋无脊椎动物粘合剂制备的天然抗氧化生物材料的研究,研究结果表明这些生物材料在组织再生和药妆品中的应用前景广阔。