Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001 Henan, China.
College of Plant Science, Tarim University/Key Laboratory of Production and Construction Corps of Agricultural Integrated Pest Management in Southern Xinjiang, Aral 843300 Xinjiang, China.
J Agric Food Chem. 2021 Dec 22;69(50):15097-15107. doi: 10.1021/acs.jafc.1c05070. Epub 2021 Dec 13.
In aphids, hormesis and symbiotic bacteria are the drivers for the development of pesticide resistance. However, the related mechanism remains unclear. Here, we evaluated the sublethal and transgenerational effects of the extensively used pyrethroid pesticide deltamethrin (DMT) on the population dynamics in and tested its influence on symbiotic bacterial communities. The leaf-dip bioassay revealed that DMT was highly toxic to , and at a low lethal concentration of DMT, the intrinsic () and finite rates of increase (λ) of the initially exposed aphids (G0) significantly decreased. Intriguingly, the , λ, and net reproductive rate () of G1 and G2 significantly increased, but the and λ decreased in G3. The adult and total preoviposition period increased in G3 but decreased in G4. Additionally, the diversity of the bacterial community decreased, while the abundance values of , Pseudomonadaceae, and Burkholderiaceae increased after 24 h of exposure to LC DMT in G0 aphids, and the latter two decreased in G1 but increased in G2. In summary, sublethal DMT has intergenerational hormesis effect on cotton aphids in G1-G2 and remarkably altered their symbiotic bacterial community and abundance. These results broaden our understanding of the relationship of hormesis and symbiotic bacteria in aphids under insecticide exposure.
在蚜虫中，毒物兴奋效应和共生细菌是导致杀虫剂抗性发展的驱动因素。然而，相关机制尚不清楚。在这里，我们评估了广泛使用的拟除虫菊酯杀虫剂溴氰菊酯（DMT）对种群动态的亚致死和世代间效应，并测试了其对共生细菌群落的影响。叶浸生物测定表明，DMT 对 具有高毒性，在低致死浓度的 DMT 下，最初暴露的蚜虫（G0）的固有（）和有限增长率（λ）显著降低。有趣的是，G1 和 G2 的 λ和净生殖率（）显著增加，但 G3 的 和 λ 降低。G3 中的成虫和总产卵前期增加，但 G4 中的成虫和总产卵前期减少。此外，暴露于 LC DMT 24 h 后，G0 蚜虫的细菌群落多样性降低，而 Pseudomonadaceae 和 Burkholderiaceae 的丰度值增加，G1 中的后两者减少，但 G2 中的增加。综上所述，亚致死 DMT 对 G1-G2 中的棉蚜具有世代间毒物兴奋效应，并显著改变了它们的共生细菌群落和丰度。这些结果拓宽了我们对昆虫暴露下毒物兴奋效应和共生细菌在蚜虫中的关系的理解。
