Huangfu Ningbo, Shang Jiao, Guo Lixiang, Zhu Xiangzhen, Zhang Kaixin, Niu Ruichang, Li Dongyang, Gao Xueke, Wang Li, Ji Jichao, Luo Junyu, Cui Jinjie
State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Pest Manag Sci. 2025 Jan;81(1):477-489. doi: 10.1002/ps.8449. Epub 2024 Sep 27.
Deltamethrin, as a highly effective and broad-spectrum insecticide, has been widely used for agricultural pest control such as Aphis gossypii worldwide. Increasing evidence has shown that despite great economic benefits brought by it, deltamethrin has also non-negligible side effects. However, the potential risks and related molecular mechanisms remain largely unclear.
Herein, the life table parameters and transcriptome sequencing analyses of the four successive aphid generations were performed to investigate the hormesis and transgenerational effects of deltamethrin on A. gossypii. The life table analysis showed that although the exposure of G0 aphid to 30% lethal concentration (LC) deltamethrin significantly reduced the net reproduction rate (R), intrinsic rate of increase (r), and fecundity of G0, but it significantly enhanced the R and fecundity of subsequent two generations (G1 and G2) of A. gossypii. Moreover, transcriptomic analyses showed that the signaling pathways related to posttranscriptional regulation (spliceosome), protein processing, longevity regulating, and cell proliferation (DNA replication, homologous recombination and non-homologous end-joining) were significantly up-regulated in G1 or G2 under LC deltamethrin treatment. Additionally, we also found that the deltamethrin-sulfoxaflor rotation of G0 and G1 still induced reproductive stimulation, but the reproductive stimulation induced by insecticides rotation treatment was significantly lower than that in the deltamethrin exposure alone.
Our study demonstrates that sublethal concentrations of deltamethrin significantly enhanced the offspring fecundity of cotton aphid. In addition, our study also reveals the transcriptional response mechanism of hormesis-induced fecundity increase, providing valuable reference for optimizing the application of deltamethrin in integrated pest management. © 2024 Society of Chemical Industry.
溴氰菊酯作为一种高效广谱杀虫剂，已在全球广泛用于防治棉蚜等农业害虫。越来越多的证据表明，尽管溴氰菊酯带来了巨大的经济效益，但其副作用也不可忽视。然而，其潜在风险和相关分子机制仍 largely 不清楚。
在此，对棉蚜连续四代进行生命表参数和转录组测序分析，以研究溴氰菊酯对棉蚜的兴奋效应和跨代效应。生命表分析表明，虽然 G0 代棉蚜暴露于 30%致死浓度（LC）的溴氰菊酯显著降低了 G0 代的净繁殖率（R）、内禀增长率（r）和繁殖力，但却显著提高了棉蚜后续两代（G1 和 G2）的 R 和繁殖力。此外，转录组分析表明，在 LC 溴氰菊酯处理下，G1 或 G2 中与转录后调控（剪接体）、蛋白质加工、寿命调节和细胞增殖（DNA 复制、同源重组和非同源末端连接）相关的信号通路显著上调。此外，我们还发现 G0 和 G1 的溴氰菊酯-氟吡呋喃酮轮用仍诱导繁殖刺激，但杀虫剂轮用处理诱导的繁殖刺激明显低于单独使用溴氰菊酯处理。
我们的研究表明，亚致死浓度的溴氰菊酯显著提高了棉蚜后代的繁殖力。此外，我们的研究还揭示了兴奋效应诱导繁殖力增加的转录反应机制，为优化溴氰菊酯在有害生物综合治理中的应用提供了有价值的参考。© 2024 化学工业协会。
