Ding Yi, Liu Guiqiong, Zeng Fanmei, Yan Yinan, Jing Haijing, Jiang Xunping
Laboratory of Sheep and Goat Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, People's Republic of China.
Anim Biotechnol. 2023 Nov;34(4):966-973. doi: 10.1080/10495398.2021.2007116. Epub 2021 Dec 14.
Androgen from the testis and weak androgens from the adrenal cortex may interact with each other and affect their synthesis and secretion due to their similar functions. The purpose of this study was to investigate the compensatory effect of adrenal in rats after immunocastration and surgical castration, and the interaction between the hypothalamic-pituitary-testis (HPT) axis and the hypothalamic-pituitary-adrenal (HPA) axis. 24 male SD rats aged 8 weeks were randomly divided into three groups and accepted treatments: surgical castration group, immunocastration group and control group. In both surgical castration and immunocastration groups, the secretion of adrenocorticotropic hormone (ACTH) and dehydroepiandrosterone (DHEA) hormones was significantly increased compared with the control group ( < 0.05). In the HPT axis of the immunocastration group, the expression was up-regulated, whereas , and expression were down-regulated ( < 0.05). The expression levels of , and genes were also significantly up-regulated ( < 0.05). In addition, in the immunocastration group, the expression of adrenal mRNA expression was decreased ( < 0.05). The expression of HPT axis genes and adrenal were up-regulated in the surgical castration group ( < 0.05). These results show that in both immunocastration and surgical castration, adrenal androgen is increased, suggesting that the adrenal gland plays a compensatory role. Moreover, it also shows that different castration treatments have effects on adrenal steroid secretion through different mechanisms.
来自睾丸的雄激素和来自肾上腺皮质的弱雄激素可能会相互作用，并因其相似的功能而影响它们的合成与分泌。本研究的目的是探讨免疫去势和手术去势后大鼠肾上腺的代偿作用，以及下丘脑-垂体-睾丸（HPT）轴与下丘脑-垂体-肾上腺（HPA）轴之间的相互作用。将24只8周龄的雄性SD大鼠随机分为三组并接受相应处理：手术去势组、免疫去势组和对照组。在手术去势组和免疫去势组中，促肾上腺皮质激素（ACTH）和脱氢表雄酮（DHEA）激素的分泌与对照组相比均显著增加（<0.05）。在免疫去势组的HPT轴中， 的表达上调，而 、 和 的表达下调（<0.05）。 、 和 基因的表达水平也显著上调（<0.05）。此外，在免疫去势组中，肾上腺 mRNA表达降低（<0.05）。手术去势组中HPT轴基因和肾上腺 的表达上调（<0.05）。这些结果表明，在免疫去势和手术去势中，肾上腺雄激素均增加，提示肾上腺发挥代偿作用。此外，还表明不同的去势处理通过不同机制对肾上腺类固醇分泌产生影响。
