Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Laboratory of Sheep and Goat Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Sci Rep. 2023 Aug 28;13(1):14025. doi: 10.1038/s41598-023-41016-5.
The content of androgen from adrenal is elevated under castration, and the mechanisms of compensatory secretion of adrenal androgen remain unknown. This study was designed to compare the transcript profiles between adrenals from noncastrated, orchiectomized and immunocastrated Yiling goats. Fifteen goats were randomly divided into three groups: pVAX-asd injection (control) group, pVAX-B2L-(GS)-kisspeptin-54-asd immunization (PBK-asd) group, and surgical castration (SC) group. Subsequently, serum was collected every two weeks after the initial immunization for hormone assays. At week 14 after immunization, adrenal glands were collected for transcriptome sequencing and qPCR. Serum testosterone concentration was significantly reduced in PBK-asd and SC group, demonstrating the effectiveness of castration. Both surgical and immunized castration resulted in adrenal hyperplasia, and thickness of adrenal cortex elevated. The specific genes involving castration were enriched in many pathways, including Steroid hormone biosynthesis pathway. The adrenocorticotropic hormone (ACTH), which promotes the production of adrenal steroids, and dehydroepiandrosterone (DHEA), a steroid hormone secreted by adrenal glands, both increased after castration. Further construction of co-expression network for transcription genes and traits (including adrenal weight and cortex thickness, ACTH and DHEA concentration) showed that the trait-related genes were enriched in multiple steroid-related pathways. These results showed that adrenal compensatory hyperplasia and androgen secretion caused by castration may involve in ACTH-induced steroid hormone synthesis.
去势后肾上腺雄激素含量升高，而肾上腺雄激素代偿性分泌的机制尚不清楚。本研究旨在比较未去势、去势和免疫去势夷陵羊肾上腺的转录谱。15 只羊随机分为三组：pVAX-asd 注射（对照）组、pVAX-B2L-（GS）-kisspeptin-54-asd 免疫（PBK-asd）组和手术去势（SC）组。随后，在初次免疫后每两周采集血清进行激素检测。免疫后 14 周，采集肾上腺进行转录组测序和 qPCR。PBK-asd 和 SC 组血清睾酮浓度显著降低，表明去势有效。手术和免疫去势均导致肾上腺增生，肾上腺皮质增厚。涉及去势的特定基因在许多途径中富集，包括甾体激素生物合成途径。促肾上腺皮质激素（ACTH）可促进肾上腺类固醇的产生，而去势后肾上腺分泌的脱氢表雄酮（DHEA）也增加。进一步构建转录基因和性状（包括肾上腺重量和皮质厚度、ACTH 和 DHEA 浓度）的共表达网络显示，与性状相关的基因在多个类固醇相关途径中富集。这些结果表明，去势引起的肾上腺代偿性增生和雄激素分泌可能涉及 ACTH 诱导的类固醇激素合成。
