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盐度对副溶血性弧菌生物膜形成和 c-di-GMP 产生的影响。

The Effect of Salinity on Biofilm Formation and c-di-GMP Production in Vibrio parahaemolyticus.

机构信息

Department of Clinical Laboratory, Nantong Third Hospital Affiliated to Nantong University, Nantong, 212006, Jiangsu, China.

School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.

出版信息

Curr Microbiol. 2021 Dec 14;79(1):25. doi: 10.1007/s00284-021-02723-2.

Abstract

Vibrio parahaemolyticus is a moderately halophilic, salt-requiring organism that exhibits optimal growth at approximately 3% salt. Thus, salinity stress is one of the most important stimuli during its lifecycle. The bacterium possesses a strong ability to form biofilms on surfaces, which are thought to be involved in protecting it from adverse environmental conditions. In the present study, salinity-dependent biofilm formation by V. parahaemolyticus was investigated by combining crystal violet staining, colony morphology, intracellular c-di-GMP quantification and quantitative PCR. The results showed that biofilm formation by V. parahaemolyticus was significantly enhanced in low salinity growth conditions and was affected by incubation time. In addition, low salinity reduced intracellular c-di-GMP degradation in V. parahaemolyticus. Transcription of genes encoding ScrABC and ScrG proteins, which are involved in intracellular c-di-GMP metabolism, was inhibited by low salinity growth conditions. Thus, reduced intracellular c-di-GMP degradation in V. parahaemolyticus in low salinity growth conditions may be mediated by repression of scrG and scrABC transcription. Taken together, these results demonstrated for the first time that salinity regulates biofilm formation and c-di-GMP production in V. parahaemolyticus.

摘要

副溶血性弧菌是一种中度嗜盐、需盐的生物体,在大约 3%的盐度下表现出最佳生长。因此,盐度应激是其生命周期中最重要的刺激之一。该细菌具有在表面形成生物膜的强大能力,生物膜被认为参与保护其免受不利环境条件的影响。在本研究中,通过结合结晶紫染色、菌落形态、细胞内 c-di-GMP 定量和定量 PCR,研究了副溶血性弧菌的盐度依赖性生物膜形成。结果表明,副溶血性弧菌在低盐生长条件下的生物膜形成显著增强,并受孵育时间的影响。此外,低盐降低了副溶血性弧菌细胞内 c-di-GMP 的降解。参与细胞内 c-di-GMP 代谢的 ScrABC 和 ScrG 蛋白编码基因的转录受到低盐生长条件的抑制。因此,副溶血性弧菌在低盐生长条件下细胞内 c-di-GMP 降解减少可能是 scrG 和 scrABC 转录抑制的结果。综上所述,这些结果首次表明盐度调节副溶血性弧菌的生物膜形成和 c-di-GMP 产生。

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