H-NS 抑制. 生物膜形成和 c-di-GMP 合成。

H-NS Represses Biofilm Formation and c-di-GMP Synthesis in .

机构信息

School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu, China.

Department of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong 226006, Jiangsu, China.

出版信息

Biomed Environ Sci. 2022 Sep 20;35(9):821-829. doi: 10.3967/bes2022.106.

DOI:10.3967/bes2022.106

PMID:36189997

Abstract

OBJECTIVE

This study aimed to investigate the regulation of histone-like nucleoid structuring protein (H-NS) on biofilm formation and cyclic diguanylate (c-di-GMP) synthesis in RIMD2210633.

METHODS

Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining, quantification of c-di-GMP, quantitative real-time polymerase chain reaction, LacZ fusion, and electrophoretic-mobility shift assay.

RESULTS

The deletion of enhanced the biofilm formation and intracellular c-di-GMP levels in RIMD2210633. H-NS can bind the upstream promoter-proximal DNA regions of , , VP0117, VPA0198, VPA1176, VP0699, and VP2979 to repress their transcription. These genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP metabolism.

CONCLUSION

One of the mechanisms by which H-NS represses the biofilm formation by RIMD2210633 may be repression of the production of intracellular c-di-GMP.

摘要

目的

本研究旨在探讨组蛋白样核小体结构蛋白（H-NS）对 RIMD2210633 生物膜形成和环二鸟苷酸（c-di-GMP）合成的调节作用。

方法

通过结晶紫染色、c-di-GMP 定量、实时定量聚合酶链反应、LacZ 融合和电泳迁移率变动分析，综合分析调控机制。

结果

缺失增强了 RIMD2210633 的生物膜形成和胞内 c-di-GMP 水平。H-NS 可结合、、VP0117、VPA0198、VPA1176、VP0699 和 VP2979 的上游启动子近端 DNA 区域，抑制其转录。这些基因编码一组与 c-di-GMP 代谢相关的具有 GGDEF 和/或 EAL 结构域的蛋白。