Kazaz Ilke Onur, Demir Selim, Kerimoglu Gokcen, Colak Fatih, Alemdar Nihal Turkmen, Akman Ahmet Ugur, Cekuc Ozan Can, Mentese Ahmet
Faculty of Medicine, Department of Urology, Karadeniz Technical University, Trabzon, Turkey.
Faculty of Health Sciences, Department of Nutrition and Dietetics, Karadeniz Technical University, Trabzon, Turkey.
J Invest Surg. 2022 May;35(5):1106-1111. doi: 10.1080/08941939.2021.2015489. Epub 2021 Dec 14.
The purpose of this study was to evaluate the possible therapeutic effect of chrysin (CHS) on testicular torsion/detorsion (T/D) injury through the mechanisms of oxidative stress and endoplasmic reticulum stress (ERS).
Eighteen male rats were divided into three groups of six subjects in each group: control, T/D and T/D + CHS (100 mg/kg). To evaluate the degree of oxidative stress, tissue malondialdehyde (MDA), total oxidant status (TOS) and total antioxidant status (TAS) levels were determined using colorimetric methods, while tissue superoxide dismutase (SOD) levels were determined using an ELISA kit. To evaluate the degree of ERS, tissue glucose regulatory protein 78 (GRP78), activating transcription factor 6 (ATF6) and C/EBP homologous protein (CHOP) levels were determined using ELISA kits. Johnsen's testicle scoring system was used for histological evaluation.
In the T/D group, it is determined that statistically significant decreasing in the levels of TAS, SOD and Johnsen score, and increasing in TOS, MDA, GRP78, ATF6 and CHOP levels compared to control group (p < 0.05). CHS administration statistically significantly restored this T/D-induced damage (p < 0.05).
This is the first study to show that CHS prevent T/D-induced testicular damage through its ERS inhibitor activity. More comprehensive studies are needed to understand the underlying mechanisms.
Supplemental data for this article is available online at https://doi.org/10.1080/08941939.2021.2015489 .
本研究旨在通过氧化应激和内质网应激（ERS）机制评估白杨素（CHS）对睾丸扭转/复位（T/D）损伤的潜在治疗作用。
将18只雄性大鼠分为三组，每组6只：对照组、T/D组和T/D + CHS组（100 mg/kg）。采用比色法测定组织丙二醛（MDA）、总氧化剂状态（TOS）和总抗氧化剂状态（TAS）水平，以评估氧化应激程度；使用酶联免疫吸附测定试剂盒测定组织超氧化物歧化酶（SOD）水平。采用酶联免疫吸附测定试剂盒测定组织葡萄糖调节蛋白78（GRP78）、活化转录因子6（ATF6）和C/EBP同源蛋白（CHOP）水平，以评估ERS程度。采用约翰森睾丸评分系统进行组织学评估。
与对照组相比，T/D组TAS、SOD水平和约翰森评分在统计学上显著降低，TOS、MDA、GRP78、ATF6和CHOP水平升高（p < 0.05）。给予CHS在统计学上显著恢复了这种T/D诱导的损伤（p < 0.05）。
这是第一项表明CHS通过其ERS抑制活性预防T/D诱导的睾丸损伤的研究。需要更全面的研究来了解其潜在机制。
本文的补充数据可在网上获取，网址为https://doi.org/10.1080/08941939.2021.2015489 。
