Molecular Cardiology Unit, Department of Biochemistry, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, 625021, India.
Center for Molecular and Translational Medicine, Georgia State University, Atlanta, GA, USA.
Mol Biol Rep. 2024 May 25;51(1):678. doi: 10.1007/s11033-024-09612-4.
Chrysin (Chy) is a naturally occurring flavonoid found in fruits, vegetables, honey, propolis, and many plant extracts that has shown notable medicinal value. Chy exhibits diverse pharmacological properties, including anti-oxidative, anti-inflammatory, anti-apoptotic, anti-cholesteremic, and cardioprotective. However, the influence of Chy in mitigating high-fat diet (HFD)-induced ER stress of rat myocardium remains unknown.
The current work intended to determine the therapeutic potential of Chy against HFD-induced endoplasmic stress-mediated apoptosis.
To evaluate the therapeutic value of Chy in HFD-induced endoplasmic stress-mediated apoptosis in the myocardium; The male wistar rats were divided into different groups; control, HFD control, HFD fed followed by Chy-treated and HFD fed followed by atorvastatin (Atv) treated rats.
When compared to the control group, the HFD-fed rats had significantly higher levels of marker enzymes such as CK-NAC and ALP, as well as lipid peroxidation and lipid profile (TC, TG, LDL, and VLDL). Chy therapy greatly reversed these marker enzymes and the lipid profile. qRT-PCR Studies showed that Chy supplementation considerably improved Nrf2 and its target genes. In addition, Chy lowered the expression of PERK, CHOP, ATF6, GRP78, and Caspase-3 genes in the heart tissue of HFD-fed rats. Immunohistochemistry results demonstrated that Chy substantially enhanced the Nrf2 and reduced PERK and Caspase3-7 protein expression in HFD-fed rats.
The current study concluded that Chy may mediate the cardioprotective effect by activating Nrf2 and inhibiting PERK signaling pathway against ER stress-mediated apoptosis induced by HFD. Therefore, supplementation with Chy could serve as a promising therapeutic target against HFD-induced ER stress-mediated cardiac complication.
白杨素（Chy）是一种天然存在的类黄酮，存在于水果、蔬菜、蜂蜜、蜂胶和许多植物提取物中，具有显著的药用价值。Chy 表现出多种药理特性，包括抗氧化、抗炎、抗凋亡、降胆固醇和心脏保护。然而，Chy 减轻高脂肪饮食（HFD）诱导的大鼠心肌内质网应激的影响尚不清楚。
本研究旨在确定 Chy 对抗 HFD 诱导的内质网应激介导的凋亡的治疗潜力。
为了评估 Chy 在 HFD 诱导的内质网应激介导的心肌细胞凋亡中的治疗价值；雄性 Wistar 大鼠分为不同组；对照组、HFD 对照组、HFD 喂养后 Chy 处理组和 HFD 喂养后阿托伐他汀（Atv）处理组。
与对照组相比，HFD 喂养组大鼠的标记酶如 CK-NAC 和 ALP 以及脂质过氧化和脂质谱（TC、TG、LDL 和 VLDL）水平显著升高。Chy 治疗极大地逆转了这些标记酶和脂质谱。qRT-PCR 研究表明，Chy 补充剂显著改善了 Nrf2 及其靶基因。此外，Chy 降低了 HFD 喂养大鼠心脏组织中 PERK、CHOP、ATF6、GRP78 和 Caspase-3 基因的表达。免疫组化结果表明，Chy 可显著增强 Nrf2 并降低 HFD 喂养大鼠中 PERK 和 Caspase3-7 蛋白的表达。
本研究得出结论，Chy 可能通过激活 Nrf2 并抑制 PERK 信号通路来介导对 HFD 诱导的内质网应激介导的凋亡的心脏保护作用。因此，Chy 的补充可能成为对抗 HFD 诱导的内质网应激介导的心脏并发症的有前途的治疗靶点。
